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Microbiology Journal

Year: 2011 | Volume: 1 | Issue: 3 | Page No.: 89-96
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Authors

Elsayed Aboulmagd

Hamdan I. Al-Mohammed

Soad Al-Badry

Keywords

Research Article

Synergism and Postantibiotic Effect of Green Tea Extract and Imipenem Against Methicillin-resistant Staphylococcus aureus

Elsayed Aboulmagd, Hamdan I. Al-Mohammed and Soad Al-Badry
This study aimed to evaluate the antibacterial activity of imipenem-green tea extract combination against methicillin-resistant Staphylococcus aureus (MRSA) isolates using disc diffusion technique, checkerboard titration method and time-killing assay. In addition, for the first time the postantibiotic effect (PAE) of green tea either alone or plus imipenem was explored. Presence of subinhibitory concentration of green tea extract (0.25xMIC) enhanced the susceptibility of ten MRSA clinical isolates to imipenem as expressed by the percentage of relative inhibition zone diameter (% RIZD) which ranged from 140 to 254%. The fractional inhibitory concentration (FIC) indices of imipenem-green tea extract combinations were from 0.156 to 0.5. For MRSA-S2 strain, combination of imipenem and green tea extract, at 0.25xMIC each, resulted in 99.99% killing after 24 h incubation. Moreover, the PAE values obtained in vitro were 1.5-2.8 h for imipenem and 2.2-3.55 h for green tea extract, while higher values of 2.9-4.7 h were obtained for the combination of imipenem and green tea extract. In conclusion, the combined effect of imipenem and green tea extract seems to be significantly synergistic and may be eligible for further evaluation in vivo against MRSA infections. In addition, extension of the PAE duration of imipenem-green tea extract combination compared with imipenem alone may have significant impact on the effect of consumption of green tea extract on the dosing regimen of imipenem.
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