Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Journal of Pharmacology and Toxicology
  Year: 2011 | Volume: 6 | Issue: 4 | Page No.: 433-439
DOI: 10.3923/jpt.2011.433.439
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Ameliorative Activity of Withania somnifera Root Extract on Paraquat-induced Oxidative Stress in Mice

Shanker K. Singh, Umesh Dimri, Meena Kataria and Priyambada Kumari

Abstract:
Paraquat (PQ), an oxidative stress inducing substance, is a herbicide which is very toxic to the animals and humans. A number of antioxidant treatments against PQ-induced oxidative stress have been tried but unfortunately, none of them has been proven to be enormously effective. In this context, attentions are needed to explore the antioxidants from the natural resources. Thus, the antioxidant activity of Withania somnifera (Ws) root extract on paraquat (PQ) induced oxidative stress in mice was investigated. The doses of 100, 250 and 500 mg kg-1 of Ws were given orally on alternate day for 30 days. PQ (3.5 mg kg-1) was injected intraperitoneally on day 15th of start of the study. On day 30th antioxidant activity of Ws was evaluated by measuring lipid peroxides (MDA) and GSH contents as well as by measuring the activities of antioxidant enzymes; superoxide dismutase (SOD) and catalase (CAT) in the blood of mice. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, creatinine and urea levels were also estimated to investigate the protective effects of Ws on liver and kidney functions. Ws remarkably alleviated the over production of MDA and improved GSH level in PQ intoxicated mice blood dose-dependently. In addition, SOD and CAT activities were improved significantly by Ws treatment. Ws had also considerably alleviated the elevated AST and ALT activities as well as the total bilirubin, creatinine and urea levels in a dose dependent manner. Ws protected the mice against paraquat-induced oxidative stress mainly through alleviating the over production of MDA and modulating the lowered GSH level, SOD and CAT activities. Thus, Ws may constitute an alternative sole or adjunct remedy against paraquat-induced oxidative stress.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Estimating the Plasma and Serum Activity Levels of Aspartate Aminotransferase and Alanine Aminotransferase, in Live Animals Using Regression Model
  •    Immunomodulatory Effect of Withania somnifera, Asparagus racemosus and Picrorhiza kurroa Roots
  •    Normalisation of Lipoprotein Phenotypes by Chromolaena odorata-Linn. in Carbon Tetrachloride Hepatotoxicity-Induced Dyslipidaemia
  •    In vitro Evaluation of Antibacterial Activities of Crude Extracts of Withania somnifera (Ashwagandha) to Bacterial Pathogens
  •    Biochemical Composition of Roots of Withania somnifera (L.) Dunal
  •    Antioxidant Effect of Arabic Gum Against Paraquat-induced Lung Oxidative Stress in Mice
  •    Tocotrienols and α-Tocopherol Reduced Acute and Chronic Lung Lipid Peroxidation Induced by Paraquat in Rats
How to cite this article:

Shanker K. Singh, Umesh Dimri, Meena Kataria and Priyambada Kumari, 2011. Ameliorative Activity of Withania somnifera Root Extract on Paraquat-induced Oxidative Stress in Mice. Journal of Pharmacology and Toxicology, 6: 433-439.

DOI: 10.3923/jpt.2011.433.439

URL: https://scialert.net/abstract/?doi=jpt.2011.433.439

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom