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Journal of Plant Sciences
  Year: 2010 | Volume: 5 | Issue: 4 | Page No.: 414-419
DOI: 10.3923/jps.2010.414.419
 
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Polyphenolics Free DNA Isolation from Different Types of Tissues of Aconitum heterophyllum Wall-endangered Medicinal Species

Nidhi Srivastava, Vikas Sharma, Barkha Kamal, A.K. Dobriyal and Vikash S. Jadon

Abstract:
The present study is aimed to isolate DNA from three types of tissues of Aconitum heterophyllum Wall using previously reported methods after modifications. Aconitum heterophyllum Wall is a vital and critically endangered herb, which needs to have its fingerprinting data for its shrewd use. Use of DNA markers with the techniques like Polymerase Chain Reaction (PCR) and molecular cloning can provide every minute detail about the subject sample. Good quality DNA is always a prerequisite in all molecular biology experiments especially PCR. As this plant contains high amounts of secondary metabolites that interfere with DNA isolation. The two methodologies described in the present study yielded good-quality, high-molecular weight DNA that is free of contaminants and colored pigments and is suitable for PCR amplification.
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How to cite this article:

Nidhi Srivastava, Vikas Sharma, Barkha Kamal, A.K. Dobriyal and Vikash S. Jadon, 2010. Polyphenolics Free DNA Isolation from Different Types of Tissues of Aconitum heterophyllum Wall-endangered Medicinal Species. Journal of Plant Sciences, 5: 414-419.

DOI: 10.3923/jps.2010.414.419

URL: https://scialert.net/abstract/?doi=jps.2010.414.419

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