Research Article

**Analysis of Cadmium-peptide Complexes in Sunflower (Helianthus annuus L.) Roots as Detected by Gel-filtration Chromatography**

Mohamed Anis Elhammadi, Radhouane Chaffai, Ali Tekitek and Ezzedine El Ferjani

Fifteen-old sunflower (Helianthus annuus L.) plants on hydroponic culture were treated with 75 μM Cd. After 3 days of Cd exposure, a decrease in growth parameters (root and stem length and leaf area) was observed. In extracts of Cd-treated roots, gel-filtration chromatography detected one Cd-binding peptide designated PC-Cd complex, with an apparent M_r of 17.8 kDa. In response to Cd, the roots exhibited a higher accumulation of non protein thiols (NPTs) compared with control plants. Moreover, phytochelatin (PC) levels, estimated from the difference between total NPTs and glutathione (GSH), increased significantly, raising the possibility that PCs play a significant role in heavy metal detoxification. The higher PC concentrations were accompanied by lower GSH concentrations.

PDF Fulltext XML References Citation

How to cite this article

Mohamed Anis Elhammadi, Radhouane Chaffai, Ali Tekitek and Ezzedine El Ferjani, 2006. Analysis of Cadmium-peptide Complexes in Sunflower (Helianthus annuus L.) Roots as Detected by Gel-filtration Chromatography. Journal of Plant Sciences, 1: 201-211.

DOI: 10.3923/jps.2006.201.211

URL: https://scialert.net/abstract/?doi=jps.2006.201.211

Journal of Plant Sciences

Article Trend

Total views 383

Search

Authors

Mohamed Anis Elhammadi

Radhouane Chaffai

Ali Tekitek

Ezzedine El Ferjani

Keywords

Research Article

**Analysis of Cadmium-peptide Complexes in Sunflower (Helianthus annuus L.) Roots as Detected by Gel-filtration Chromatography**

How to cite this article

Related Articles

Leave a Comment