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Journal of Medical Sciences

Year: 2013 | Volume: 13 | Issue: 7 | Page No.: 546-554
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Authors

Hanafey F. Maswada

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Research Article

Assessment of Total Antioxidant Capacity and Antiradical Scavenging Activity of Three Egyptian Wild Plants

Hanafey F. Maswada
The present study was undertaken to evaluate in vitro antioxidant and antiradical activities of the hydro-ethanolic (50%) extract of the above and underground parts of three wild geophytic species, Asparagus stipularis, Cyperus capitatus and Stipagrostis lanata. Total Antioxidant Capacity (TAC) using phosphomolybdenum method and antiradical scavenging activity using 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) and hydrogen peroxide methods and Total Phenolics (TP), Total Flavonoids (TF) contents of plant extracts were assessed. Results revealed that, the extract of C. capitatus (underground part) had the highest values of TP (163.35 mg tannic acid equivalent/g extract) and TF (12.31 mg catechol equivalent/g extract) while; the lowest values of TP (14.33 mg TAE g-1) and TF (0.53 mg CE g-1) were recorded in the extract of A. stipularis (underground part). The same trend was found in case of Total Antioxidant Capacity (TAC) and antiradical scavenging activity, where C. capitatus (underground part) exhibited the highest TAC (21.21 mg TAE g-1 extract) and antiradical activity with IC50 = 0.061 and 0.167 mg mL-1 for DPPH and H2O2 radicals, respectively. However, all plant extracts displayed strong scavenging activity against DPPH except the underground part of A. stipularis extract, exhibited moderate activity. Furthermore, the highest H2O2 scavenging activity was detected in the extracts of C. capitatus followed by A. stipularis and then S. lanata. This study concluded that, the investigated geophytic plants could be utilized as good sources of natural antioxidants for medicinal and commercial uses especially the underground parts of C. capitatus.
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