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Journal of Medical Sciences
  Year: 2007 | Volume: 7 | Issue: 3 | Page No.: 361-367
DOI: 10.3923/jms.2007.361.367
 
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Enhancement of Antibody Immune Response to a Toxoplasma gondii SAG1-Encoded DNA Vaccine by Formulation with Aluminum Phosphate

K. Solhjoo, F. Ghaffari Far, A. Dalimi-Asl and Z. Sharifi

Abstract:
In the present study, we evaluated aluminum phosphate as an adjuvant of SAG1-encoded DNA vaccine against T. gondii in BALB/c mice. Anti-T. gondii IgG values increased markedly in the pcSAG1 and pcSAG1+AlPO4 groups, which were significantly higher than those of control groups (p< 0.05). Although pcSAG1+AlPO4 elicited IgG antibody values were greater than that of the sera of mice immunized with pcSAG1 alone, there was no statistically significant difference between these two groups (p>0.05). In spite of remaining alive of one mouse more than 45 days in pcSAG1+AlPO4 group after challenge with T. gondii tachyzoites, all of the experimental mice died and the survival time of the mice immunized with pcSAG1+AlPO4 was markedly longer than those of control and pcSAG1 groups (p< 0.05). All mice in the control groups died within 7-9 days after challenge, whereas the average survival time of pcSAG1 and pcSAG1+AlPO4 groups were up to 14 and 21 days after infection, respectively. Indeed its low cost, ease of use and safety record, these findings indicate that the AlPO4 adjuvant can be used to combine DNA-based vaccination to prime an enhanced and balanced specific immunity.
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How to cite this article:

K. Solhjoo, F. Ghaffari Far, A. Dalimi-Asl and Z. Sharifi, 2007. Enhancement of Antibody Immune Response to a Toxoplasma gondii SAG1-Encoded DNA Vaccine by Formulation with Aluminum Phosphate. Journal of Medical Sciences, 7: 361-367.

DOI: 10.3923/jms.2007.361.367

URL: https://scialert.net/abstract/?doi=jms.2007.361.367

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