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Research Journal of Microbiology

Year: 2017 | Volume: 12 | Issue: 2 | Page No.: 128-136
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Authors

Hamsah

Widanarni

Alimuddin

Munti Yuhana

M. Zairin Junior

Keywords

Research Article

Bacterial Population, Activity of Enzymes and Growth Rate of Pacific White Shrimp Larvae Administered Pseudoalteromonas piscicida and Mannan-oligosaccharides through Bioencapsulation of Artemia sp.

Hamsah, Widanarni, Alimuddin, Munti Yuhana and M. Zairin Junior
Background and Objective: The application of probiotics, prebiotics and synbiotics is commonly applied in juvenile and adult shrimp administered through artificial feed, while in larvae, it has still been limited. This study aimed to evaluate growth performance, the total intestinal bacteria, the activity of enzymes and the survival rate of Pacific white shrimp (Litopenaeus vannamei) administered Pseudoalteromonas piscicida 1Ub, mannan-oligosaccharides and synbiotic (the combination of P. piscicida 1Ub and mannan-oligosaccharides) through bio-encapsulation of Artemia sp. Materials and Methods: Bio-encapsulation of Artemia sp. was done by adding P. piscicida 1Ub 106 CFU mL–1, mannan-oligosaccharides 12 mg L–1 and synbiotic (P. piscicida 1Ub 106 CFU mL–1 and mannan-oligosaccharides 12 mg L–1) to the rearing medium of Artemia sp., for 4 h. The administration of the enriched Artemia sp. to the shrimp larvae was done from mysis 3 to Post Larvae (PL) 12. The body length and the body weight of Pacific white shrimp larvae were observed at the beginning and the end of the study, while RNA/DNA ratio, the activity of enzymes, survival rate and total bacteria of shrimp larvae were analyzed at the end of the study. Results: The results showed that daily growth rate, absolute length, RNA/DNA ratio, the activity of enzymes, survival rate and total bacteria of shrimp larvae administered probiotic, prebiotic and synbiotic were higher (p<0.05) than the control. The synbiotic treatment gave the best results in daily growth rate (24.39±0.31%), absolute length (13.00±0.50 mm), RNA/DNA ratio (0.6369±0.0094 μg mL–1), the activity of enzymes (protease 0.033±0.0007, lipase 0.047±0.0010, amylase 0.853±0.008, mannanase 0.148±0.004 U mL–1 min–1), survival rate (92.67±1.26%) and total bacteria (6.7×107 CFU larvae–1). Conclusion: The administration of P. piscicida 1Ub, mannan-oligosaccharides and synbiotic through bio-encapsulation of Artemia sp., effectively improved the growth performance of Pacific white shrimp larvae with the best results demonstrated by the synbiotic treatment.
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How to cite this article

Hamsah, Widanarni, Alimuddin, Munti Yuhana and M. Zairin Junior, 2017. Bacterial Population, Activity of Enzymes and Growth Rate of Pacific White Shrimp Larvae Administered Pseudoalteromonas piscicida and Mannan-oligosaccharides through Bioencapsulation of Artemia sp.. Research Journal of Microbiology, 12: 128-136.

URL: https://scialert.net/abstract/?doi=jm.2017.128.136

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