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Research Journal of Microbiology
  Year: 2013 | Volume: 8 | Issue: 2 | Page No.: 92-100
DOI: 10.3923/jm.2013.92.100
 
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Characterization and Inhibition Effect of Conyza Leaf Extract (Pluchea dioscoridis) on Lipase Enzyme Produced by Aspergillus niger, Isolated from Otomycosis Disease

M.A. Metwally, H.M. Mubarak, A.M. Gamea and N.A. El Zawawy

Abstract:
This study reported the inhibition of Aspergillus niger lipase enzyme by Conyza leaf extract which considered an effective tool to prevent fungal invasion into the external ear canal causing otomycosis. Lipase enzyme was extracted from otomycotic A. niger, precipitated by ammonium sulphate and purified by Sephadex G-100 chromatography. The mode of Conyza leaf extract inhibitory action on lipase was detected by preparing both different substrate and Conyza concentrations. The results showed that 30% ammonium sulphate fractionation gave maximum lipase activity 59.2 U mL-1 and the highest lipase activity of Sephadex G-100 chromatography purified sample was found to be 79.1 U mL-1 . The specific activity was 87.9 U mg-1 proteins with purification fold of 8.7. Electrophoretic analysis for the purified enzyme indicated one subunit of molecular weight of 45 kDa. Conyza extract acted as irreversible competitive lipase inhibitor with inhibition constant (kis = 6.2 mg mL-1). The results obtained in the present study suggest that Conyza extract can be used to inhibit A. niger growth that causes otomycosis.
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How to cite this article:

M.A. Metwally, H.M. Mubarak, A.M. Gamea and N.A. El Zawawy, 2013. Characterization and Inhibition Effect of Conyza Leaf Extract (Pluchea dioscoridis) on Lipase Enzyme Produced by Aspergillus niger, Isolated from Otomycosis Disease. Research Journal of Microbiology, 8: 92-100.

DOI: 10.3923/jm.2013.92.100

URL: https://scialert.net/abstract/?doi=jm.2013.92.100

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