Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Research Journal of Microbiology
  Year: 2012 | Volume: 7 | Issue: 2 | Page No.: 114-122
DOI: 10.3923/jm.2012.114.122
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Differential Responses of Marine Sediment Bacteria Pseudomonas stutzeri Strain VKM014 to Chromate Exposures
P.V. Bramhachari, J. Ravichand , K.V. Deepika , P. Yalamanda and K.V. Chaitanya

Abstract:
The aim of the present study was to isolate and characterize the bacterial strain of a chromium-contaminated site and to evaluate the differentially expressed proteins of strain VKM014, when exposed to varied concentrations of heavy metal chromium. Sediment samples were collected from the Port of Kakinada situated on the Southern Part of East Coast of India and plated on Nutrient agar medium containing chromate. Strain VKM014 produced both mucoid biofilm and biosurfactant. The strain VKM014 which could tolerate high levels of chromate (0.75 mM) was further selected for strain characterization, growth kinetics and expression of chromate-induced proteins. Based on morphological, biochemical characteristics and phenogram, the isolate was tentatively grouped under Pseudomonas sp. and identified as Pseudomonas stutzeri based on 16S rRNA gene sequence analysis (GenBank EF079450). This study results indicated that chromate upshock resulted in induction/enhancement of two unidentified 25 and 28 kDa induced proteins in the cell extract of the VKM014 following the exposures to 0.2 and 0.5 mM of chromate. In contrast, changes at biochemical level (induced proteins) are the first detectable responses to environmental perturbations and so the sensitive indicators of pollution. This study demonstrates new links between biosurfactant production, differential subpopulation response and metal exposures and involvement of certain enzymes in chromate resistance.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Detoxification of Cr (VI) by Bacillus cereus S-6
  •    Epigenetic Effects of Carcinogens
  •    Aerobic Chromate Reducing Bacillus cereus Isolated from the Heavy Metal Contaminated Ennore Creek Sediment, North of Chennai, Tamil Nadu, South East India
  •    Efficacy of Indigenous Bacillus Species in the Removal of Chromium from Industrial Effluent
How to cite this article:

P.V. Bramhachari, J. Ravichand , K.V. Deepika , P. Yalamanda and K.V. Chaitanya , 2012. Differential Responses of Marine Sediment Bacteria Pseudomonas stutzeri Strain VKM014 to Chromate Exposures. Research Journal of Microbiology, 7: 114-122.

DOI: 10.3923/jm.2012.114.122

URL: https://scialert.net/abstract/?doi=jm.2012.114.122

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom