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The Potency of Endophytic Fungi of Turmeric (Curcuma longa L.) in Biotransformation of Curcumin Compounds in Various Media |
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T.K. Prana,
J. Srikandace,
E. Sumitro and D. Wulandari |
Abstract:
With the aim of investigating the potency of endophytic fungi in biotransforming curcumin, a total of 45 endophytic fungi have been isolated from rhizomes of turmeric plant (Curcuma longa L.) obtained from several turmeric cultivation centres in West Java and Central Java areas, i.e., Bogor, Cibinong, Parung, Serang, Yogyakarta, Cilacap and Banyumas. The isolates was selected for their potency in biotransforming curcumin into its derivative compounds in 4 different media, namely Potato Dextrose Broth (PDB), Czapek medium, Shyntetic Low Nutrient (SLN) and Saboraud media. Fermentation process was carried out at room temperature with shaking for 14 days after addition of 0.01% w/v curcumin. Extraction method of fermentation products was done applying partition method using ethyl-acetate solvent (1:1). The extracts obtained were then analyzed using TLC (Thin Layer Chromatography) chloroform: metanol eluent (10:1). The Biotransformation Products compound (BTP) was confirmed through High Performance Liquid Chromatography (HPLC). The results indicated that 4 out of 45 isolates were able to biotransform curcumin into other compound. The 4 isolates were no. Cl.Pa.4F (fom Parung), Cl.Bel.5F (from Serang ), Cl.Tg.3F (from Yogyakarta) all in PDB medium, whilst isolate no.Cl.Bk.5F (from Serang) showed the ability in Saboraud medium. However, further test indicated that only Cl.Bel.5F isolate showed stability in biotransforming curcumin. It was confirmed that BTP could be detected beginning on day 5 and reached optimum at day 10. The BTP has lower color intensity and more polar compare to curcumin.
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How to cite this article:
T.K. Prana, J. Srikandace, E. Sumitro and D. Wulandari, 2010. The Potency of Endophytic Fungi of Turmeric (Curcuma longa L.) in Biotransformation of Curcumin Compounds in Various Media. Research Journal of Microbiology, 5: 1189-1198.
DOI: 10.3923/jm.2010.1189.1198
URL: https://scialert.net/abstract/?doi=jm.2010.1189.1198
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