
	Journal of Fisheries and Aquatic Science
	Year: 2012 \| Volume: 7 \| Issue: 6 \| Page No.: 422-430 DOI: 10.3923/jfas.2012.422.430

Evaluation of Bacillus subtilis Effect as Probiotic on Hematological Parameters of Rainbow Trout, Oncorhynchus mykiss (Walbaum) Following Experimental Infection with Streptococcus iniae

Maryam Kamgar and Masood Ghane

Abstract: In this study, Bacillus subtilis is used for control of streptococcosis with the agent of Streptococcus iniae in rainbow trout. The experience was carried out in 2 groups (Control (c) and Treatment (T)) and 3 replicates. In control group, probiotic was not applied in diet but in treatment group, Bacillus subtilis was administered in feed at a concentration of 10⁷cells g^-1. At 45th day, 0.1 mL intraperitoneally injection of S. iniae with 2x10⁷ cells mL^-1 dosage was given to both groups and were checked for the rest of the survey duration (2 weeks). At the end of the time which took about two months, blood samples were caught for hematological experiments for realizing the effect of B. subtilis feeding on resistance of fish against S. iniae infection. After injection of S. iniae, there was no significant difference among T and C groups considering parameters such as erythrocyte count, hemoglobin, hematocrit, Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC) and percent of monocyte (p>0.05). But significant difference was seen in the leucocyte count, percent of lymphocyte and neutrophile in both groups (p<0.05). The leucocyte count and percent of lymphocyte was higher in T group and neutrophile percent was lower in comparison with the control. The results of the present study indicate that B. subtilis can be used as an agent for the control of streptococcosis in rainbow trout hatchery and culture farms for decreasing economical disasters.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Evaluation of a New Egyptian Probiotic by African Catfish Fingerlings

•	Effect of Biogen^® and Myco-Ad^® on the Growth Performance of Common Carp (Cyprinus carpio) Fed a Mycotoxin Contaminated Aquafeed

•	Effect of Feeding Bacillus sp. As Probiotic Bacteria on Growth of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man)

•	Effect of Probiotic Combination Fermented Soybean Meal on Growth Performance, Lipid Metabolism and Immunological Response of Growing-Finishing Pigs

•	Effect of Commercial Probiotics on Large Scale Culture of Black Tiger Shrimp Penaeus monodon (Fabricius)

How to cite this article:

Maryam Kamgar and Masood Ghane, 2012. Evaluation of Bacillus subtilis Effect as Probiotic on Hematological Parameters of Rainbow Trout, Oncorhynchus mykiss (Walbaum) Following Experimental Infection with Streptococcus iniae. Journal of Fisheries and Aquatic Science, 7: 422-430.

DOI: 10.3923/jfas.2012.422.430

URL: https://scialert.net/abstract/?doi=jfas.2012.422.430

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ProQuest

Science Alert