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Journal of Biological Sciences

Year: 2022 | Volume: 22 | Issue: 1 | Page No.: 24-33
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Authors

Afolake Atinuke Olanbiwoninu

Racheal Oluwayemisi Fashogbon

Racheal Oluwayemisi Fashogbon

Kayode Olaitan Owoyemi

Keywords

Background and Objective: 'Iru ’ is a traditional condiment obtained by spontaneous alkaline fermentation of African locust bean (Parkia biglobosa) seeds. B. subtilis is the most predominant microorganism associated with the fermentation of 'Iru' which antimicrobial substances such as peptides, antibiotics and bacteriocins. This work is aimed at isolation, identification, screening of the antimicrobial activity and partial purification of the Bacteriocin-Like Inhibitory Substances (BLIS) from B. subtilis obtained from' Iru'. Materials and Methods: Twenty-four 'Iru' samples were collected from different 'Iru' sellers in different markets in Oyo town. Isolation was done using the Luria-Bertani Agar medium. Identification was carried out using cultural, morphological, biochemical and molecular characterization. Agar well diffusion method was used for the antimicrobial activity. Partial purification of BLIS was done by Ammonium sulphate precipitation. The technological properties and the tolerance to bile salt were also determined. Identification of bioactive compounds was done using Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC). Results: A total of 59 bacteria were isolated, 13 were identified to be Bacillus subtilis. Five Bacillus subtilis (BSAAJ3, BSFAJ3, BSDAJ3, BSFAK2 and BSAAW3) inhibited Staphylococcus aureus while B. subtilis (BSFAK4) inhibited E. coli. B. subtilis (BSFAK4) had the highest protease, lipase, esterase, hemolytic potential and tolerance to bile salt. The GC-MS analysis identified 23 bioactive compounds while the HPLC analysis revealed the presence of ribonuclease, cytochrome c, a-chymotrypsinogen and lysozyme as the proteins present. Conclusion: These studies revealed that the BLIS obtained from BSFAK4 had the potential to inhibit a wide spectrum of pathogens which will be very useful in the pharmaceutical industry.
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How to cite this article

Afolake Atinuke Olanbiwoninu, Racheal Oluwayemisi Fashogbon and Kayode Olaitan Owoyemi, 2022. Antimicrobial Activity of Bacteriocin-Like Inhibitory Substances Produced by Bacillus subtilis Isolated from “Iru”. Journal of Biological Sciences, 22: 24-33.

DOI: 10.3923/jbs.2022.24.33

URL: https://scialert.net/abstract/?doi=jbs.2022.24.33

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