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Journal of Biological Sciences
  Year: 2011 | Volume: 11 | Issue: 5 | Page No.: 374-380
DOI: 10.3923/jbs.2011.374.380
 
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Evaluation of Natural Exudate Gum from Sterculia urens as Gelling Agent in Culture Media for In vitro Regeneration of Rough Lemon (Citrus jambhiri Lush.) Shoot Tips

Balwinder Singh, Amritpal Kaur and Jaspreet Singh

Abstract:
Natural exudate gum (sterculia gum) has been successfully used as gelling agent in culture media with an aim to reduce the production cost of tissue culture raised plants. Shoot tips were cultured on agar and sterculia gum gelled media to study the difference in shoot and root regeneration response. Initially, shoot tips were cultured on agar gelled Murashige and Skoog (MS) medium supplemented with different concentrations of 6-Benzyl amino purine (BAP), Kinetin (KN) and Naphthalene acetic acid (NAA). Maximum percentage of cultures showing shoot regeneration (63.19%) was observed on agar gelled MS medium containing BAP 1.5, KN 0.5 and NAA 0.5 mg L-1. Sterculia gum and agar were then evaluated at different concentrations and combinations. The media gelled with sterculia gum was equally transparent as that of liquid medium. The shoot regeneration response on media gelled with 25 and 30 g L-1 sterculia gum were 49.99 and 61.11% respectively. Regenerated shoots were rooted on agar gelled MS medium supplemented with different concentrations of Indole-3-Acetic Acid (IAA), NAA and Indole -3-butyric acid (IBA, 0.5-2.5 mg L-1). Maximum rooting response (56.94%) was observed with IBA 2.0 mg L-1. Same medium when evaluated for rooting response using sterculia gum (30 g L-1) as gelling agent, 56.24% cultures showed root regeneration. The difference in regeneration response obtained with agar (8 g L-1) and sterculia gum (30 g L-1) was statistically insignificant. This study indicates that sterculia gum can be used as alternative gelling agent in place of agar for the development of cost effective micropropagation protocol.
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How to cite this article:

Balwinder Singh, Amritpal Kaur and Jaspreet Singh, 2011. Evaluation of Natural Exudate Gum from Sterculia urens as Gelling Agent in Culture Media for In vitro Regeneration of Rough Lemon (Citrus jambhiri Lush.) Shoot Tips. Journal of Biological Sciences, 11: 374-380.

DOI: 10.3923/jbs.2011.374.380

URL: https://scialert.net/abstract/?doi=jbs.2011.374.380

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