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Journal of Biological Sciences

Year: 2011 | Volume: 11 | Issue: 4 | Page No.: 314-319
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Authors

K. Khucharoenphaisan

U. Puangpetch

K. Puttaraksa

K. Sinma

Keywords

Research Article

Grouping of Actinomycetes Isolated from Termites using Biochemical Character

K. Khucharoenphaisan, U. Puangpetch, K. Puttaraksa and K. Sinma
Actinomycetes are a group of prokaryotic organisms belonging to Gram-positive bacteria and play an important ecological role in recycling substances in the nature. To determine possible established correlation between isolated actinomycetes and its biochemical degradation (xylan, soluble starch, cassava, protein, lipid, uric acid, carboxymethyl cellulose and avicel degradation) and oxidation property (guaiacol), the actinomycetes were isolated from Termes sp. The dendogram was generated from UPGMA analysis with FreeTree software. In this work, 45 strains of actinomycetes were isolated from guts of Termes sp. Among these, 44 isolated strains could degrade protein, soluble starch and cassava starch. Twenty-three strains degraded uric acid and xylan. The isolated strains that able to degrade avicel and oxidize lignin were rare. The morphological character showed the variety of aerial hyphae and spore forming in each strain. The dendogram was constructed based on biodegradation activity of tested strains. The tested strains were classified into 5 clusters. Cluster 1, cluster 2, cluster 3, cluster 4 and cluster 5 contained 14, 9, 9, 9 and 4 isolated strains, respectively. The actinomycetes strains showed a similar biodegration activity within a same cluster. This result indicated the relation between biodegradation activity and actinomycetes strains. Dendogram based on the biodegradation activity was found to be an efficiencient tool for grouping purpose.
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