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Journal of Biological Sciences

Year: 2010 | Volume: 10 | Issue: 6 | Page No.: 468-479
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Authors

N. Hanafi

S.Z. Mansour

Keywords

Research Article

Antitumor Efficacy of Salenostemma Argel and/or γ-Irradiation Against Ehrlich Carcinoma

N. Hanafi and S.Z. Mansour
The present study aims to evaluate the antitumor effect of aqueous extract of Salenostemma Argel (SA) leaves either alone or combined with 6 Gy of γ-radiation exposure against Ehrlich Carcinoma (EC) bearing mice. SA extract was gavages at the doses of 15 mg kg-1 b.wt. SA gavages to experimental animals 24 h and 7 days after Tumor Inoculation (ATI). γ-radiation exposure was done 7 days ATI as a single dose. In the present study aqueous extract of SA chemosensitivity, growth of transplantable murine tumor, apoptotic and necrotic detection in tumor tissue were done. Histopathological examination, biochemical parameters such as lipid peroxidation (MDA), glutathione content (GSH), superoxide dismutase (SOD) and catalase (CAT) activities for the murine tumor and liver tissues were also examined. SA activates tumor cell death after two weeks. SA significantly decrease tumor volume and recorded high and wide zones of apoptotic tumor cells in tumor tissue either alone or combined with γ- irradiation. SA and γ-irradiation resultant a non significant change in MDA and a non significant change in GSH level and CAT activity in tumor tissue either alone or combined with γ- irradiation. SA ameliorates MDA and significantly ameliorates GSH level and CAT and SOD activity in liver tissue of EC-bearing mice. Histopathologically SA and/or γ-irradiation represented large areas of apoptosis, hydropic degeneration and nuclear debris in tumor tissue section. However, in liver tissue SA treatment represent increase in Kupffer cells and decrease of inflammatory cells. SA and γ-irradiation represented normal appearance of liver tissue section. It is concluded that SA and/ or γ-irradiation represent antitumor activities.
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