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Journal of Applied Sciences
  Year: 2010 | Volume: 10 | Issue: 24 | Page No.: 3307-3312
DOI: 10.3923/jas.2010.3307.3312
 
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Cellulases Production in Palm Oil Mill Effluent: Effect of Aeration and Agitation

K. Fadzilah and M.D. Mashitah

Abstract:
Effect of aeration (0.5, 1.0 and 1.5 vvm) and agitation rate (100, 300 and 500 rpm) on cellulase production in submerged culture of Pycnoporus sanguineus was studied in a 2.5 L stirred-tank bioreactor using Palm Oil Mill Effluent (POME) as a substrate. Maximum cell biomass (3.16 g L-1) and cellulase activity (0.1748 FPU mL-1) was obtained at aeration rate of 1.0 vvm and agitation speed of 300 rpm. Volumetric mass transfer coefficient (kLa) was found to be dependent on aeration and agitation rate, with maximum kLa (124.2 h-1) attained at 300 rpm and 1.5 vvm.
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How to cite this article:

K. Fadzilah and M.D. Mashitah, 2010. Cellulases Production in Palm Oil Mill Effluent: Effect of Aeration and Agitation. Journal of Applied Sciences, 10: 3307-3312.

DOI: 10.3923/jas.2010.3307.3312

URL: https://scialert.net/abstract/?doi=jas.2010.3307.3312

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