
	Journal of Applied Sciences
	Year: 2010 \| Volume: 10 \| Issue: 24 \| Page No.: 3307-3312 DOI: 10.3923/jas.2010.3307.3312

Cellulases Production in Palm Oil Mill Effluent: Effect of Aeration and Agitation

K. Fadzilah and M.D. Mashitah

Abstract: Effect of aeration (0.5, 1.0 and 1.5 vvm) and agitation rate (100, 300 and 500 rpm) on cellulase production in submerged culture of Pycnoporus sanguineus was studied in a 2.5 L stirred-tank bioreactor using Palm Oil Mill Effluent (POME) as a substrate. Maximum cell biomass (3.16 g L^-1) and cellulase activity (0.1748 FPU mL^-1) was obtained at aeration rate of 1.0 vvm and agitation speed of 300 rpm. Volumetric mass transfer coefficient (k_La) was found to be dependent on aeration and agitation rate, with maximum k_La (124.2 h^-1) attained at 300 rpm and 1.5 vvm.

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How to cite this article:

K. Fadzilah and M.D. Mashitah, 2010. Cellulases Production in Palm Oil Mill Effluent: Effect of Aeration and Agitation. Journal of Applied Sciences, 10: 3307-3312.

DOI: 10.3923/jas.2010.3307.3312

URL: https://scialert.net/abstract/?doi=jas.2010.3307.3312

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