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Journal of Applied Sciences

Year: 2009 | Volume: 9 | Issue: 10 | Page No.: 1936-1942
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Authors

H. Khazali

M. Behzadfar

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Research Article

Effect of Orexin Infusion into Third Ventricle on the GnRH and LH Secretions in the Prepubertal Rat

H. Khazali and M. Behzadfar
The goal of this study was to determine whether orexin affects gonadotropin releasing hormone (GnRH) and Luteinizing Hormone (LH) secretions in the prepubertal male and female rats. Forty prepubertal rats were randomly divided into 4 groups. Animals in group 1 and 2 were male and 3 and 4 were female. Animals in group 1 and 3 received infusions of 1 μg orexin and group 2 and 4 received infusions of 2 μg orexin into their third ventricle. Blood samples were collected from jugular veins, every 30 min from 4 h before the first infusion of orexin until 4 h after the last orexin infusion. Infusions of 1 and 2 μg orexin significantly (p<0.01) decreased the mean plasma concentrations and pulse amplitudes of GnRH and LH in prepubertal female animal. Also, infusions of 1 and 2 μg orexin significantly (p<0.05) decreased the mean plasma concentrations and pulse amplitudes of GnRH and LH in prepubertal male animal, but this decrease were lower in than female prepubertal rat. Infusions of 1 and 2 μg orexin did not change the mean plasma concentrations of FSH in the animal of all groups. Infusions of 1 and 2 μg, orexin significantly (p<0.01) decreased the glucose levels of the prepubertal female animals. The conclusion of this experiment indicated that orexin may negatively affect the GnRH and LH in the prepubertal rats with negative energy balance, but not in those with the positive energy balance.
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