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Journal of Applied Sciences
  Year: 2008 | Volume: 8 | Issue: 14 | Page No.: 2599-2605
DOI: 10.3923/jas.2008.2599.2605
 
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Polypyrrole-Based Xanthine Oxidase Potentiometric Biosensor for Hypoxanthine

A.T. Lawal and S.B. Adeloju

Abstract:

A biosensor is developed for the determination of hypoxanthine (Hx) by entrapment of XOD and a mediator, ferrocene or potassium ferrocyanide, (K4Fe(CN)6, into a PPy film during galvanostatic film formation. The optimum conditions for the formation of PPy-XOD-Fe(CN)64- film include 0.4 M pyrrole, 6.2 U mL-1 XOD, 40 mM K4Fe(CN)6, polymerisation period of 200 sec and an applied current density of 0.5 mA cm-2. Potentiometric modes of detection, was investigated. The potential for the potentiometric biosensing of Hx was 0.0 mV vs. Ag/AgCl (3 M KCl) in 0.05 M phosphate buffer. It is revealed that potentiometric detection was sensitive and gave a wider linear concentration range. The PPy-XOD potentiometric biosensor was successfully used for the determination of fish freshness.

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How to cite this article:

A.T. Lawal and S.B. Adeloju, 2008. Polypyrrole-Based Xanthine Oxidase Potentiometric Biosensor for Hypoxanthine. Journal of Applied Sciences, 8: 2599-2605.

DOI: 10.3923/jas.2008.2599.2605

URL: https://scialert.net/abstract/?doi=jas.2008.2599.2605

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