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International Journal of Zoological Research
  Year: 2011 | Volume: 7 | Issue: 2 | Page No.: 190-200
DOI: 10.3923/ijzr.2011.190.200
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Standardization and Optimization of mtDNA Isolation and Molecular Genetic Analysis of D-loop Region in Animal Natural Fibres

Priyanka P. Rane and S.S. Barve

Increase in demand for animal natural fibres in recent years for the production of high quality textile products has resulted in the adulteration and false declaration of these fibres causing heavy financial loss. Fibres are expensive due to limited feedstock and less fibre production. To keep up with the demand these fibres are adulterated with less expensive fibres viz., wool to give special effect to the fabric. To control false declaration, there is a need for fibre identification and to ascertain blend composition. Though Scanning Electron Microscopy is generally used for fibre analysis but this method is time consuming, expensive and the reliability of results depend on the expertise of the microscopist. Hence, there is a need for reliable and economical method to characterize these fibres and to study composition of each animal fibre in blends. The aim of the present study was mitochondrial DNA extraction from animal natural fibres in untreated and blends. The modified protocol includes addition of Proteinase K, Dithioerythritol individually in each tube and final extraction with phenol: chloroform: isoamyl alcohol, amplification of D-loop region using species specific and mammalian specific primers. We observed that with species specific primers, it was possible to study inter species variation but the blends could be detected if there was prior knowledge about the fibres in blends. With mammalian specific primers we could study blends and differentiate between fibres from sheep breeds but inter species variation was difficult. It can be concluded that mtDNA analysis can be used to differentiate animal fibres and control adulteration.
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How to cite this article:

Priyanka P. Rane and S.S. Barve, 2011. Standardization and Optimization of mtDNA Isolation and Molecular Genetic Analysis of D-loop Region in Animal Natural Fibres. International Journal of Zoological Research, 7: 190-200.

DOI: 10.3923/ijzr.2011.190.200






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