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International Journal of Virology

Year: 2007 | Volume: 3 | Issue: 3 | Page No.: 107-116
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Authors

T.Z. Salem

S.M. El-Gamal

A.S. Sadik

Keywords

Research Article

Use of Helper Component Proteinase Gene to Identify a New Egyptian Isolate of Watermelon Mosaic Potyvirus

T.Z. Salem, S.M. El-Gamal and A.S. Sadik
In this study, an Egyptian isolate of potyvirus known to be zucchini yellow mosaic potyvirus (ZYMV) was isolated from leaves of the cultivar Giza 1 watermelon plants which cultivated in Agricultural Experimental Research Station, Sids, Beni Suef, Egypt. The double antibody sandwich (DAS)-enzyme-linked immunosorbent assay (ELISA) was carried out using polyclonal antibodies specific to ZYMV. The helper component proteinase gene (Hc-pro) of WMV was isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) using primers based on the DNA sequence of ZYMV virus, strain KR-PA. The blast searches identify the Hc-pro sequence as a part of Water Melon Mosaic Potyvirus (WMV). The sequence analysis showed that the isolated Hc-pro gene belongs to a new isolate of WMVs and its DNA sequence and deduced amino acid were compared with the Hc-pro of the French, China and Pakistan isolates. This study showed that the Egyptian isolate is closer to Pakistan isolate than French and China isolates, however, the phylogenetic tree showed that using one gene is not enough to relate these isolates to each other.
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