Abstract:
The aim of this research is to study the resistance response to Alternaria blight by different varieties of wheat alongwith growth stages of plant. Resistance response to Alternaria blight by different varieties of wheat namely HUW 612, K 508, K 0708, HD 3003, K 402, HUW 468 and PBW 343 revealed that all the varieties give variable response on disease severity. Among the varieties, minimum disease severity was recorded in K 0708 with the value of 11, 21 and 30% at flowering, dough and hard dough stage. Biochemical analysis of leaves of different varieties of wheat revealed that the variable amount of phenol and soluble protein content. The maximum with 3.12, 2.98, 2.59 and 2.60 mg g-1 of total phenol and 31.52, 30.79, 29.73 and 29.73 mg g-1 of soluble protein content was found in K 0708 at seedling, flowering, dough and hard dough stage, respectively. The banding pattern of soluble protein showed that quantitative and qualitative differences are existing among the variety. Protein profiling by SDS-PAGE revealed that the variety, HUW 612 has the maximum number of bands with 23. The presence or absence of bands in protein profiling might be responsible for resistance to Alternaria blight in wheat. The correlation co-efficient between disease severity and soluble protein content at different stage of wheat varieties revealed that there was negative correlation showing (r) -0.4212 (flowering), -0.4221 (dough) and -0.4592 (Hard dough) on the varieties HUW 612. The regression equation of soluble protein and disease severity showed that higher regression value, lower disease incidence. The variety K 0708 has the correlation coefficient value as (r) -0.4310 at flowering stage -0.4410 at dough stage and -0.4420 at the hard dough stage, resulting highest response of resistance to Alternaria triticina.
|
