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International Journal of Plant Pathology
  Year: 2011 | Volume: 2 | Issue: 3 | Page No.: 136-143
DOI: 10.3923/ijpp.2011.136.143
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Variability among Isolates of Fusarium oxysporum f. sp. chrysanthemi Pathogenic to Chrysanthemum

P.K. Singh and Vijay Kumar

Fusarium oxysporum f. sp. chrysanthemi (FOC) is one of the most wide spread and destructive pathogen, causing infection to Chrysanthemum plants throughout the world. Qualitative evaluation of 10 FOC isolates was done for their ability to produce fusaric acid, using thin layer chromatography. A variation in FOC isolates was observed and isolate FO-1, FO-2 and FO-3 were very strong producers of fusaric acid. Isolate FO-1 produced highest biomass in liquid medium and radial diameter on solid medium and FO-2 was the slowest. Growth of FOC isolates was recorded maximum on Potato Dextrose Broth (PDB), followed by Czapex Dox Broth (CDB) and FSM-Liquid. All the isolates of FOC showed significantly (p<0.01) higher growth on pH 5 followed by 7, 9, 3 and 11. FOC isolates growth was highest at temperature of 25°C followed by 30, 20, 15, 35 and 40°C. None of FOC isolates showed radial growth at 40 and 45°C. Results of this study has revealed that a temperature of 25°C, pH of 5.0 and Potato dextrose medium was most suitable for the growth of FOC isolates.
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How to cite this article:

P.K. Singh and Vijay Kumar, 2011. Variability among Isolates of Fusarium oxysporum f. sp. chrysanthemi Pathogenic to Chrysanthemum. International Journal of Plant Pathology, 2: 136-143.

DOI: 10.3923/ijpp.2011.136.143






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