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International Journal of Plant Breeding and Genetics
  Year: 2016 | Volume: 10 | Issue: 2 | Page No.: 79-90
DOI: 10.3923/ijpbg.2016.79.90
 
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Assessing the Relative Efficacy of Polyester Pollination Bags and Crossing Tents and Isolation Chambers for Seed Harvest in Miscanthus Crosses

C. Hayes and D.S. Virk

Abstract:
Background and Objective: Pollination bags and tents play a crucial role in artificial hybridisation in plant breeding. They offer protection against unwanted foreign pollen and bird damage and provide ambient conditions for hybrid seed development. Methodology: Five hundred and sixty four inter- and intra-specific crosses were made in Miscanthus using three types of pollination bags in glasshouse and isolation chamber conditions in experiment 1. Another 16 crosses were made in a duraweb® tent and isolation chamber under external and glasshouse conditions in experiment 2. Data on quantitative traits were analysed using Z-test and analysis of variance techniques. Results: The success rate of crosses with duraweb® bags was the highest (45%) which exceeded the glassine bags by 15% and orchard type bags by 7%. The success rate for the intra-specific crosses was 47% that was 13% higher than the inter-specific crosses. The seed set rate with duraweb® crossing tents was much higher than in isolation chambers. The average seed number in tents was 82% higher than in isolation chambers in glasshouse conditions. Better seed set rate in duraweb® bags and tents resulted from a better control on the temperature and humidity within them. The isolation chambers had higher temperature and humidity than the duraweb® tents and hence showed a lower seed number. Both duraweb® bags and tents were reusable following an autoclave cycle and were resistant to slug damage. Conclusion: Both duraweb® bags and crossing tents showed greater efficacy in hybrid seed production by providing an ambient environment for seed development.
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How to cite this article:

C. Hayes and D.S. Virk, 2016. Assessing the Relative Efficacy of Polyester Pollination Bags and Crossing Tents and Isolation Chambers for Seed Harvest in Miscanthus Crosses. International Journal of Plant Breeding and Genetics, 10: 79-90.

DOI: 10.3923/ijpbg.2016.79.90

URL: https://scialert.net/abstract/?doi=ijpbg.2016.79.90

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