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International Journal of Pharmacology
  Year: 2019 | Volume: 15 | Issue: 7 | Page No.: 823-828
DOI: 10.3923/ijp.2019.823.828
 
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Protective Effect of Obovatol Against MCF-7 Human Breast Adenocarcinoma Cells via Inducing Apoptosis and Cell Cycle Arrest

Lihong Yang , Ying Zhang and Xuehui Yu

Abstract:
Background and Objective: Breast cancer are a frequently diagnosed cancer in a female with a high mortality. This cell line study was planned to investigate the chemo protective efficiency of obovatol (OBO) in MCF-7 human breast adenocarcinoma cells. Materials and Methods: The MCF-7 cell treated with various concentrations of OBO (10, 25, 50 and 100 μM) to evaluate the anti-proliferative/ cytotoxicity study by MTT assay, pro-apoptotic activity by assessing caspase-3/9 (apoptotic markers) and protein expression of Bcl2 and Bax as well as cell cycle arresting property (cycle phases) by flow cytometry. Results: The MCF-7 cells supplemented with increased concentration of OBO displayed significant cytotoxicity/anti-proliferative activity with increased DNA fragmentation. Whereas, cell cycle phase analysis revealed considerable cell arrest after the addition of OBO by improving G0/G1and S phase. Moreover, the activities of caspase-3 and 9 were notably increased after treatment with OBO as well as the protein expression of Bax (pro-apoptotic protein) were markedly upregulated with a suppressed protein expression of Bcl2 (anti-apoptotic protein) were noted in MCF-7 administered with OBO in a dose-dependent manner. Conclusion: Overall, OBO showed potent chemopreventive activity by demonstrating its anti-proliferative, apoptosis and cell cycle arrest properties in a concentration-dependent fashion.
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How to cite this article:

Lihong Yang, Ying Zhang and Xuehui Yu, 2019. Protective Effect of Obovatol Against MCF-7 Human Breast Adenocarcinoma Cells via Inducing Apoptosis and Cell Cycle Arrest. International Journal of Pharmacology, 15: 823-828.

DOI: 10.3923/ijp.2019.823.828

URL: https://scialert.net/abstract/?doi=ijp.2019.823.828

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