Effects of 1-O-hexyl-2, 3, 5-trimethylhydroquinone in Carbon Tetrachloride-induced Hepatic Apoptosis with a Possible Relationship to Naofen

Research Article

Effects of 1-O-hexyl-2, 3, 5-trimethylhydroquinone in Carbon Tetrachloride-induced Hepatic Apoptosis with a Possible Relationship to Naofen

Koji Tsunekawa, Jun An, Lei Huang, Toshiaki Nonami, Tatsuro Koide, Fumio Kondo, Hiroshi Nishikawa, Tokutaro Miki, Satoru Sugiyama and Naohisa Ishikawa

1-O-hexyl-2, 3, 5-trimethylhydroquinone (HTHQ), a synthesized vitamin E derivative, is a superoxide scavenger which retains the original property of vitamin E as a natural lipophilic chain-breaking antioxidant. The present study was undertaken to evaluate the effects of HTHQ on hepatocyte apoptosis, using the carbon tetrachloride (CCl₄)-induced rat hepatic cirrhosis model. We also clarified the changes in expression of naofen, previously reported as an intracellular WD-repeat protein associated with apoptosis. CCl₄ was injected twice a week and HTHQ was mixed in the drinking water, with daily intake being allowed. Rats were divided into four groups: a CCl₄-treated group, a CCl₄ with daily intake of HTHQ group and solvent- or HTHQ-treated groups without CCl₄. Within eight weeks in situ hybridization studies showed that naofen-positive hepatocytes elicited positive reactions in the TUNEL assay. CCl₄ induced TUNEL-positive staining and also enhanced naofen mRNA expression in the livers. HTHQ inhibited both TUNEL staining and naofen expression caused by CCl₄. Furthermore, the levels of naofen mRNA expression in CCl₄-treated rat liver tissue were significantly reduced by treatment with HTHQ (1.90±0.18 vs. 1.29±0.08, p<0.01). HTHQ may inhibit both apoptosis and naofen expression in CCl₄-treated rats, thus reducing liver cirrhosis.

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Koji Tsunekawa, Jun An, Lei Huang, Toshiaki Nonami, Tatsuro Koide, Fumio Kondo, Hiroshi Nishikawa, Tokutaro Miki, Satoru Sugiyama and Naohisa Ishikawa, 2012. Effects of 1-O-hexyl-2, 3, 5-trimethylhydroquinone in Carbon Tetrachloride-induced Hepatic Apoptosis with a Possible Relationship to Naofen. International Journal of Pharmacology, 8: 434-439.

DOI: 10.3923/ijp.2012.434.439

URL: https://scialert.net/abstract/?doi=ijp.2012.434.439

International Journal of Pharmacology

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Authors

Koji Tsunekawa

Jun An

Lei Huang

Toshiaki Nonami

Tatsuro Koide

Fumio Kondo

Hiroshi Nishikawa

Tokutaro Miki

Satoru Sugiyama

Naohisa Ishikawa

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Research Article

Effects of 1-O-hexyl-2, 3, 5-trimethylhydroquinone in Carbon Tetrachloride-induced Hepatic Apoptosis with a Possible Relationship to Naofen

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