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International Journal of Pharmacology
  Year: 2011 | Volume: 7 | Issue: 8 | Page No.: 821-828
DOI: 10.3923/ijp.2011.821.828
 
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Vitamin E: An Antioxidant Therapy to Protect Endosulphan Induced Follicular Toxicity

R.K. Sharma, P.K. Chauhan and A. Fulia

Abstract:
Endosulphan is a xenoestrogen that imitates the effect of estrogens, causing reproductive and developmental abnormalities in mammals. The aim of the present investigation was to analyze the effect of vitamin E in rescue of degenerating changes induced by endosulphan in granulosa cells of goat Capra hircus in vitro. On the basis of colour and texture normal follicles (3-5 mm in diameter) were selected for the tissue culture. The follicles were divided into two groups (one control+two experimental groups). Experimental group (A) was exposed with 100 nmol mL-1 endosulphan concentration. Experimental group (B) was exposed with 100 nmol mL-1 endosulphan as well as supplemented with 100 μmol L-1 concentration of vitamin E (α-Tocopherol). Harvesting was carried out after 1 h, 4 h and 8 h of exposure. Control was run simultaneously along with all the experimental groups. Endosulphan at dose level 100 nmol mL-1 induced a decline in cell diameter from 7.5±0.0456 in control to 4.5±0.1024, 3.7±0.1001 and 3.2±0.1154 μm after exposure of 1, 4 and 8 h, respectively but in case of endosulphan supplemented with vitamin E, there was less decline in cell diameter that was 6.4±0.1235, 4.8±0.1809 and 4.1±0.0809 μm after exposure of 1, 4 and 8 h, respectively. Endosulphan induced atretogenic changes like hyalinization of granulosa cells, crinkled and wavy membranes and pycnosis and thus affects the functions in adult goat due to the oxidative stress. Vitamin E treatment at dose level 100 μmol L-1 in experimental group (B) these atretogenic changes were milder and restore the normal structure of granulosa cells.
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How to cite this article:

R.K. Sharma, P.K. Chauhan and A. Fulia, 2011. Vitamin E: An Antioxidant Therapy to Protect Endosulphan Induced Follicular Toxicity. International Journal of Pharmacology, 7: 821-828.

DOI: 10.3923/ijp.2011.821.828

URL: https://scialert.net/abstract/?doi=ijp.2011.821.828

COMMENTS
26 December, 2011
Satyabroto Banerji:
The toxic effects of all pesticides, including Endosulfan, occur due to exposure of quantities above the validated safety levels. Furthermore, there are acute effects apart from the ones referred to in this paper. Therefore, the safe and judicious use of pesticides, including Endosulfan, is relevant. Contingent actions matter for acute toxicity. Deliberate chronic exposure is unethical and should not be supported. I am surprised that an Ethics Committee has permitted this work.
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