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International Journal of Pharmacology

Year: 2011 | Volume: 7 | Issue: 3 | Page No.: 400-404
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Authors

Chanchal Deep Kaur

Swarnlata Saraf

Keywords

Research Article

Photochemoprotective Activity of Alcoholic Extract of Camellia sinensis

Chanchal Deep Kaur and Swarnlata Saraf
The main antioxidant polyphenols present in Camellia sinensis (green tea) are known as epicatechins which function as anti-inflammatory or anticarcinogenic agents. There are various evidences in the literature for the protective effects that green tea polyphenols exert on the skin’s immune system on oral and topical application. The aim of this study was to produce green tea extract and to evaluate its photochemoprotective ability. The alcoholic extract of green tea leaves was prepared by continuous hot extraction method using Soxhlet apparatus. The antioxidant activity of the extract was assessed by reducing power estimation method in which the antioxidant activity of extract was compared with that of standard (ascorbic acid). The individual antioxidant activity obtained was 51.12±1.8% and combined antioxidant activity (ascorbic acid with extract) showed additive synergistic effect as compared to that of standard. In vitro SPF was determined according to the spectrophotometric method where alcoholic dilution of green tea extract was prepared and in vitro photoprotective activity was studied by UV spectrophotometeric method in the range of 290-320 nm. The extract produced high absorbances at 290-320 nm wavelength range and SPF obtained was 18.10±0.05 which collectively confirmed the photoprotective activity of green tea polyphenols. The higher antioxidant activity and sun protection factor of green tea extract could be utilized in the preparation of photoprotective cosmetic formulations which could prevent the skin with harmful effects of ultra violet radiations.
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