Subscribe Now Subscribe Today
Science Alert
Curve Top
International Journal of Pharmacology
  Year: 2011 | Volume: 7 | Issue: 2 | Page No.: 248-256
DOI: 10.3923/ijp.2011.248.256
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

In vitro and in vivo Antioxidant Activity of Aqueous Leaves Extract of Leonotis leonurus (L.) R. Br.

S.O. Oyedemi and A.J. Afolayan

The present study investigated antioxidant activities of Leonotis leonurus extract both in vivo and in vitro to provide scientific basis to traditional usage of this plant. The in vitro antioxidants activity was evaluated by determining ferric reducing power, total flavonoids, phenolics, flavonols and proanthocyanidins contents using standard assay methods. The ability of the extract to scavenge 2, 2 diphenyl-2-picrylhydrazyl (DPPH), Nitric Oxide (NO), 2, 2-azinobis [3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt were also assessed using spectroscopic method. Oral administration of L. leonurus extract at the doses of 125, 250 and 500 mg kg-1 body weight was evaluated in Wistar rats induced with carbon tetrachloride (CCl4) for 7 days. The extract effectively increased the percentage inhibition of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). However, lipid peroxidation was significantly decreased in CCl4 treated rats with the extract when compared with the diabetic control rats. The plant extract (0.8 mg mL-1) scavenge DPPH.+ ABTS.+ and NO.+ by inhibiting 72.48, 78.02 and 70.43% of the radicals, respectively. The reducing power of the extract was found to be concentration dependent. In addition, the extracts yielded higher phenolics content followed by flavonoids, proanthocyanidins and flavonols. A positive linear correlation was established between these polyphenols and the free radical scavenging activities. The results obtained from this study indicate that L. leonurus is a potential source of antioxidants and thus could prevent many radical related diseases.
PDF Fulltext XML References Citation Report Citation
  •    Polyphenolic Contents and Free Radical Scavenging Potential of Extracts from Leaves of Ocimum americanum L.
  •    Preliminary Studies on the In vitro Antioxidant Potential and Vitamin Composition of Selected Dietary Fruits Consumed in Alice region of South Africa
  •    Pharmacological Studies of Ten Medicinal Plants Used for Analgesic Purposes in Congo Brazzaville
  •    Evaluation of Free Radical Scavenging Activity of Pandanus odoratissimus
  •    Total Phenolic Content and Free Radical Scavenging Activity of Malva parviflora L. (Malvaceae)
  •    Antioxidant and Antibacterial Properties of Lecaniodiscus cupanioides
How to cite this article:

S.O. Oyedemi and A.J. Afolayan, 2011. In vitro and in vivo Antioxidant Activity of Aqueous Leaves Extract of Leonotis leonurus (L.) R. Br.. International Journal of Pharmacology, 7: 248-256.

DOI: 10.3923/ijp.2011.248.256






Curve Bottom