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International Journal of Pharmacology

Year: 2010 | Volume: 6 | Issue: 3 | Page No.: 183-191
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Authors

U.P. Chaudhari

A. Raje

N.D. Trivedi

A.N. Bhandari

Keywords

Research Article

Antidepressant like Effect of N(G)-Nitro-L-Arginine Methyl Ester

U.P. Chaudhari, A. Raje, N.D. Trivedi and A.N. Bhandari
The aim of the present study was to evaluate the antidepressant action of Nitric Oxide (NO) synthase inhibitor L-NAME (N(G)-nitro-L-arginine methyl ester) as well as its interaction with the conventional antidepressant drugs and to delineate the possible mechanism of its antidepressant action using forced swimming model in mice. Also, synergistic action of L-NAME and the drugs which act on the receptors involved in depression was studied. The antidepressant activity was assessed in Forced Swim Test in S.Mice. Total immobility period was recorded during a 6 min time. The L-NAME (10 mg kg-1, i.p.) produced a reduction in immobility period in force Swim Test. L-NAME (10 mg kg-1, i.p.) reversed the clonidine (150 μg kg-1, i.p.) induced depressions in mice were observed. Clonidine induced increased immobility time was significantly reverse by the treatment with L-LAME. Whereas reserpine induced depression is significantly reversed by L-NAME. Pretreatment of haloperidol (2 mg kg-1, i.p.) shown high immobility time which partially reversed by treatment of L-NAME. Pindolol (10 mg kg-1, i.p.) fully block the antidepressant activity of L-NAME. Antidepressant-like effect of L-NAME (5 mg kg-1) prevented by pretreatment with L-arginine in mice Force Swimming Test (FST). In addition, treatment of mice with methylene blue (10 mg kg-1 i.p.) (direct inhibitor of both Nitric Oxide Synthase (NOS) and guanylate cyclase) potentiates the effect of L-NAME (2.5 mg kg-1) in FST. From above results it is concluded that the L-NAME produced antidepressant-like activity through adrenergic system and L-arginine-NO-cGMP pathway.
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