
	International Journal of Dairy Science
	Year: 2007 \| Volume: 2 \| Issue: 3 \| Page No.: 275-280 DOI: 10.3923/ijds.2007.275.280

Bile and Acid Tolerance of Lactobacillus plantarum KCA-1: A Potential Probiotical Agent

Kingsley C. Anukam and Tara E Koyama

Abstract: A previously characterized biosurfactant-producing Lactobacillus plantarum KCA-1 isolated from the vagina of a healthy premenopausal woman was used in this study. Lactobacillus plantarum KCA-1 was tested for survival in different concentrations (0, 0.05, 0. 1, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0) of porcine and oxgall bile extract, incubated at 37°C for 48 h. Acid tolerance of the culture was studied by inoculating the organism (10⁵CFU mL^-1) in de Man Rogas Sharpe (MRS) broth at different pH values adjusted with HCl and incubated at 37°C for 48 h. Lactobacillus plantarum strain KCA-1 that produced biosurfactant needed for preventing adhesion of pathogens was tolerant to oxgall bile. There was an increase of 5 log cycles from the initial bacterial inocula (1x10⁵cfu mL^-1) to 1x10¹⁰cfu mL^-1 at 0.05 and 0.1% concentrations of oxgall bile and 4 log cycle increase (1x10⁹cfu mL^-1) at 0.5% oxgall concentration. There was a consistent reduction of 1 log cycle of every 0.5% increase of the oxgall for tested concentrations (0.5 to 4.0%). In addition, Lactobacillus plantarum KCA-1 was tolerant at pH 2.5 for 48 h of incubation as there was 2.3 to 3.8 increase in the log cycles. There was a corresponding increase in the number of cells from 1x10⁵ CFU to 1x10⁹ CFU mL^-1 at pH 4.5. The tested potential probiotic strain Lactobacillus plantarum KCA-1 exhibited a satisfactory degree of acid and bile tolerance. The findings suggests that the strain may be a promising candidate for use as a dietary adjunct, but more studies are needed.

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How to cite this article:

Kingsley C. Anukam and Tara E Koyama , 2007. Bile and Acid Tolerance of Lactobacillus plantarum KCA-1: A Potential Probiotical Agent. International Journal of Dairy Science, 2: 275-280.

DOI: 10.3923/ijds.2007.275.280

URL: https://scialert.net/abstract/?doi=ijds.2007.275.280

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