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International Journal of Cancer Research
  Year: 2018 | Volume: 14 | Issue: 2 | Page No.: 92-99
DOI: 10.3923/ijcr.2018.92.99
 
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Profile of miR-10b Expression and Metastases-related Genes in BC and Fibro Adenoma in West Sumatera

Dessy Arisanty , Yanwirasti , Jamsari Jamsari , Wirsma A. Harahap and Daan Khambri

Abstract:
Background and Objective: miR-10b is one of oncogenic miRNA first described in promoting metastases in Breast Cancer. The aim of the study was to investigate the expression of miR-10b and the expression of metastases-induced genes in BC (BC) and fibroadenoma (FA) in West Sumatra. Materials and Method: LNATM primer enhancer set was used to identify the miR-10b expression as a relative quantification. miR-16 was used as an endogenous control with a relative median expression of miR-10b at 2–ΔΔCt. The expression of metastases-related genes was performed by an absolute quantification method. Results: The statistical differences between miR-10b and the expression of genes were determined by t-student test to interpret the expression of BC and FA tissue (p<0.005). The expression of miR-10b in BC was lower than FA (endogenous control). Relative median of miR-10b expression in BC was 8.51 times lower than FA. Low expression of miR-10b in BC was associated with tissue grading. The expression of metastases-related gene; RhoC, TIMP2 and MMP2 were lower in BC than FA. miR-10b expression differed between BC and FA. Conclusion: RhoC, TIMP2 and MMP2 expression caused cancer cell whether to be metastasized or not. miR-10b is a potential marker to predict BC cell which start to be aggressive.
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How to cite this article:

Dessy Arisanty, Yanwirasti , Jamsari Jamsari, Wirsma A. Harahap and Daan Khambri, 2018. Profile of miR-10b Expression and Metastases-related Genes in BC and Fibro Adenoma in West Sumatera. International Journal of Cancer Research, 14: 92-99.

DOI: 10.3923/ijcr.2018.92.99

URL: https://scialert.net/abstract/?doi=ijcr.2018.92.99

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