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International Journal of Cancer Research

Year: 2015 | Volume: 11 | Issue: 2 | Page No.: 52-66
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Authors

R. Offei- Oknye

J.L. Patterson

L.T. Walker

J. Boateng

M. Verghese

Keywords

Phytochemicals are known to have antioxidative, antibacterial, antifungal, anti-inflammatory and anticancer properties. In this study, HepG2 hepatoma cells were incubated after 12 and 24 h with 80% methanol extracts of processed ginger: Oven dried, sun dried, freeze dried and fresh ginger (control). The objectives of this study were to investigate the chemopreventive potential of ginger extracts in hepatoma cell lines (induction of cytotoxicity and apoptosis) and to determine the effects of ginger extracts on selected detoxification and anti-oxidative enzymes: Glutathione-S-transferase, Glutathione reductase, Glutathione peroxidase, Superoxide dismutase, Catalase activity and Glutathione in cell lines (hepatoma). Processed ginger extracts induced higher cytotoxicity (%) in HepG2 (SD 60 μg mL-1 [92.89]). Induction of cytotoxicity (%) was higher after 24 h of incubation compared to 12 h. Histone related DNA fragmentation (enrichment factor), an indicator of apoptosis was higher after 12 h compared 24 h of incubation except with cell incubated with FD (240 μg mL-1). Detoxification and antioxidative enzymes studied generally showed a higher induction after 24 h compared to 12 h of incubation in SOD, GR, CAT, GPx and GSH (antioxidant substrate). These findings indicate that ginger may exerts its chemopreventive effects by its cytotoxic activity and the induction of detoxification and antioxidative enzymes.
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