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International Journal of Cancer Research

Year: 2008 | Volume: 4 | Issue: 2 | Page No.: 41-51
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Authors

J. Boateng

M. Verghese

L.T. Walker

L. Shackelford

C.B. Chawan

Keywords

Research Article

Antitumor and Cytotoxic Properties of Dry Beans (Phaseolus sp. L.): An in vitro and in vivo Model

J. Boateng, M. Verghese, L.T. Walker, L. Shackelford and C.B. Chawan
The aim of this study was to examine antitumor effects of Kidney Beans (KB) on Azoxymethane (AOM) induced colon cancer and the cytotoxic effects of KB extracts on colon cancer cell lines (CaCO2). For ACF study, 12 Fisher 344 male rats were fed AIN-93G control diet (C) (n = 6) and 20% KB (n = 6), for 13 week. In the EPTM two groups of rats (n = 14) were fed AIN-93G control diet (C) and 20% KB. All rats received two s/c injections of AOM at 7 and 8 week of age at the rate of 16 mg kg-1 body weight in saline. At 17 week (ACF) and 45 week of age (End-point) all rats were killed by CO2 asphyxiation. For lactate dehydrogenase (LDH) assay, cells were incubated (24 and 48 h) with selected concentrations (0.5-25 mg/100 mL) of KB extract. Total number of ACF was 158 and 72 for groups fed C and 20% KB. Tumor size (mm) and TBR for C and 20% KB were 6.50; 1.16 and 3.8; 1.44, respectively. LDH release (%) in CaCO2 cells after 24 and 48 h incubation with KB extracts ranged from 13.8 to 62.8 and 23.5 to 84.1, respectively. Feeding KB significantly (p<0.05) reduced the incidence of AOM induced colon tumorigenesis and KB extracts demonstrated cytotoxic effects on colon cancer cell lines (CaCO2).
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