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International Journal of Biological Chemistry
  Year: 2012 | Volume: 6 | Issue: 4 | Page No.: 130-135
DOI: 10.3923/ijbc.2012.130.135
 
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Isolation of Stigmasterol and β-Sitosterol from Chloroform Extract of Leaves of Corchorus fascicularis Lam.

A.P. Rajput and T.A. Rajput

Abstract:
The present study aimed to identify and characterize the active principles from the leaves of Corchorus fascicularis Lam. For isolation of compounds, the dried leaves powder of Corchorus fascicularis Lam. was subjected to cold maceration with chloroform as solvent and subjected to chromatography. Two compounds were isolated and purified by chloroform. Mass spectrum of CEC-1 and CEC-2 showed a parent molecular ion peak at m/z 412 which correspond to molecular formula C29H48O and 414 corresponds to C29H50O. In lH-NMR spectrum of CEC-1, H-3 proton appeared as a triplet of a double doublet (tdd) at δ 3.51 and H-6 olefinic proton showed a multiplet at δ 5.14. Two olefinic protons appeared downfield at δ 4.16 (m) and δ 4.14 (m), six methyl protons also appeared at δ 1.21, δ 1.17, δ 1.03, δ 0.99, δ 0.97 and δ 0.90 (3H each, s, CH3).The 1H-NMR data of CEC-2 it was seen that H-3 proton appeared at δ 3.52 as a triplet of a doublet of doublet and H-6 olefinic proton showed a multiplet at δ 5.35. Moreover, six methyl protons appeared at δ 1.26, δ 1.17, δ 1.03, δ 1.00, δ 0.91 and δ 0.90 for H-19, H-18, H-26, H-27, H-21, H-29, respectively for methyl group. From physical, chemical and spectral characteristics CEC-1 and CEC-2 were concluded as Stigmasterol and β-Sitosterol.
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How to cite this article:

A.P. Rajput and T.A. Rajput, 2012. Isolation of Stigmasterol and β-Sitosterol from Chloroform Extract of Leaves of Corchorus fascicularis Lam.. International Journal of Biological Chemistry, 6: 130-135.

DOI: 10.3923/ijbc.2012.130.135

URL: https://scialert.net/abstract/?doi=ijbc.2012.130.135

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