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International Journal of Biological Chemistry

Year: 2011 | Volume: 5 | Issue: 6 | Page No.: 342-351
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Authors

O. Gonchar

I. Mankovska

Keywords

Research Article

Activity and Expression of the Antioxidant Enzyme MnSOD in the Mitochondria During Prolonged Hypoxia/hyperoxia Exposure

O. Gonchar and I. Mankovska
The purpose of this study was to determine the influence of repetitive moderate hypoxia and hyperoxia (H/H) on the specific activity of manganese superoxide dismutase (MnSOD), MnSOD protein synthesis and mRNA MnSOD expression as well as on the pro-/antioxidant homeostasis in lung and heart mitochondria of rats exposed to acute severe hypoxia. It was shown that H/H pretreatment 5 cycles of 5 min hypoxia (10% O2 in N2) alternated with 5 min hyperoxia (30% O2 in N2) daily for two weeks reduced the acute hypoxia-induced lipid peroxidation, increased the GSH/GSSG ratio, the level of GSH and the activity of glutathione peroxidase in lung and heart mitochondria. In lung mitochondria H/H training resulted in increases in both the activity of MnSOD and protein synthesis. In heart mitochondria adaptation to moderate H/H enhanced the MnSOD protein expression and decreased MnSOD the acute hypoxia-induced hyperactivation. At the same time, in mitochondria of both tissues MnSOD mRNA expression was lower than in mitochondria normoxic rats. This study indicates that the increased level of MnSOD-antioxidative defense as well as the maintenance of glutathione redox status in mitochondria from H/H-treated rats is of the fundamental importance for the oxidative stress keeping at the tolerable level and confirms that mitochondrial protection during H/H may be mediated through the modulation of mitochondrial antioxidant levels.
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How to cite this article

O. Gonchar and I. Mankovska, 2011. Activity and Expression of the Antioxidant Enzyme MnSOD in the Mitochondria During Prolonged Hypoxia/hyperoxia Exposure. International Journal of Biological Chemistry, 5: 342-351.

URL: https://scialert.net/abstract/?doi=ijbc.2011.342.351

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