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International Journal of Botany

Year: 2011 | Volume: 7 | Issue: 2 | Page No.: 183-188
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Authors

Norhayati Daud

Rosna Mat Taha

Nor Nafizah Mohd Noor

Hasimah Alimon

Keywords

Plant tissue culture techniques often require optimization for cost reducing by substitute the culture medium with kitchen necessity. This study conducted to investigate various commercial starches namely; cassava flour, rice flour, corn flour and potato starch to be alternatives gelling agents in culture medium preparation. MS basal medium was prepared without plant regulator supplemented with different combinations of alternative gelling agent with Oxoid technical agar. After 8 weeks in culture, the highest of shoot regeneration were obtained on cultured in MS medium containing 40 g L-1 corn flour (10.20±9.17), 60 g L-1 cassava flour (40.00±6.73), 40 g L-1 rice flour (16:00±12.45) and the combination of 40 g L-1 potato starch plus 2 g L-1 agar (27.20±8.59). ANOVA test shows there were significant differences interactions effects (p<0.05) in terms of number of shoot regenerated between the gelling agents used (40, 50 and 100 g L-1) in combination of agar. On the other hand, no significant different interaction effects at combination of 60 g L-1 gelling agent. The results showed that the combination of alternative gelling agent with agar or gelling agent alone successfully regenerated shoots from the stem segment explants of Celosia sp. No physiological effects were observed on shoots regeneration on Celosia sp. Applications of these alternative gelling agents in tissue culture media should be considered to achieve the optimum benefit for in vitro shoots regeneration. These options are cheaper than control one and could be used for low cost alternatives.
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