Science Alert
 
Blue
   
Curve Top
International Journal of Botany
  Year: 2011 | Volume: 7 | Issue: 1 | Page No.: 103-107
DOI: 10.3923/ijb.2011.103.107
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Influence of PGRS for the in vitro Plant Regeneration and Flowering in Portulaca oleracea (L.): A Medicinal and Ornamental Plant

Madan Mohan Sharma, Abhijeet Singh, Roop Narayan Verma, Deevan Zulfikar Ali and Amla Batra

Abstract:
In vitro flowering system was developed to conquer the setback associated with flower development in in vivo condition. For this, an efficient, reliable and quick regeneration protocol has been developed for Portulaca oleracea L. using nodes of ~ 1.0-1.5 cm size. Each node procured from young, healthy shoots from garden raised plants. Multiple shoot formation (5.13±0.34) was recorded within two weeks on MS nutrient medium fortified with kinetin (0.5 mg L-1). Shoots were multiplied by subculturing on the same medium after 21 days. On first subculture, in vitro flower buds developed, when the developed shoots were maintained for another two weeks on the same medium along with gibberellic acid (0.2 mg L-1). When these flower bud containing shoots were transferred to MS medium containing Indole-3-butyric acid (0.25 mg L-1), in vitro roots emerged and the flower buds underwent anthesis. The rooted plantlets were hardened and successfully established in earthen pots with 70% success rate. The present research illustrates in vitro flowering system to get rid off problems associated with flower growth and development.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Plant Regeneration Through Direct Shoot Formation From Sago Palm (Metroxylon sagu Rottb.) Leaf Explants
  •    Effect of Cycocel and B-nine (Growth Retardants) on Growth and Flowering of Erysimum marshallii (Henfr.) Bois
  •    Effect of Amino Acids and Growth Regulators on Indirect Organogenesis in Artemisia vulgaris L.
  •    Micropropagation of Bacopa monnieri through Protoplast
  •    Effect of Plant Growth Regulators on Callus Induction and Plantlet Regeneration of Bitter Apple (Citrullus colocynthis) from Stem Explant
  •    An Efficient In vitro Hardening Technique of Tissue Culture Raised Plants
How to cite this article:

Madan Mohan Sharma, Abhijeet Singh, Roop Narayan Verma, Deevan Zulfikar Ali and Amla Batra, 2011. Influence of PGRS for the in vitro Plant Regeneration and Flowering in Portulaca oleracea (L.): A Medicinal and Ornamental Plant. International Journal of Botany, 7: 103-107.

DOI: 10.3923/ijb.2011.103.107

URL: https://scialert.net/abstract/?doi=ijb.2011.103.107

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 



Curve Bottom