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International Journal of Botany
  Year: 2006 | Volume: 2 | Issue: 3 | Page No.: 307-312
DOI: 10.3923/ijb.2006.307.312
 
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Bioactivities of Endophytic Actinomycetes from Selected Medicinal Plants in the World Heritage Site of Saint Katherine, Egypt

Sahar El-Shatoury, Hesham Abdulla, Omnia El-Karaaly, Waleed El-Kazzaz and Ahmed Dewedar

Abstract:
Endophytic actinomycetes were isolated from surface-sterilized living aerial parts of four medicinal plants, Artemisia herba-alba, Echinops spinosus, Mentha longifolia and Ballota undulate. A simplified method for selective recovery of actinomycetes from internal plant tissues is described. Successful recovery was achieved on the media: starch casein agar, 1/10 diluted starch casein agar, tap water yeast extract agar and MG-plant extract agar. A total of 41 isolates were obtained, belonging to different genera with a prevalence of Streptomyces; tentatively identified according to microscopic examination and amino acid composition of the whole cell hydrolystates. The highest number of isolates was recovered from Mentha longifolia and represented 34% of the isolates. Organic extracts of all cultures were assayed for cytotoxicity against Artemia salina and for antimicrobial activities against 4 reference bacterial strains, 6 clinical bacterial cultures and two clinical fungal cultures. Variable activities were obtained with different actinomycete isolates; the highest activity was against the clinical culture Gardnerella vaginitis and the reference strain Shigella boydii ATCC 9207, being represented in 29% of the isolates in both cases. For the first time, the isolation of actinomycetes from Saint Katherine wild plants is reported and their potential use as novel source of bioactive compounds is discussed.
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How to cite this article:

Sahar El-Shatoury, Hesham Abdulla, Omnia El-Karaaly, Waleed El-Kazzaz and Ahmed Dewedar, 2006. Bioactivities of Endophytic Actinomycetes from Selected Medicinal Plants in the World Heritage Site of Saint Katherine, Egypt. International Journal of Botany, 2: 307-312.

DOI: 10.3923/ijb.2006.307.312

URL: https://scialert.net/abstract/?doi=ijb.2006.307.312

COMMENTS
26 May, 2012
Ajit Kumar Passari ( JRF ):
This paper is very good. I am also interested to isolate the endophytic actinomycetes from medicinal plants. So, Please send this paper to my email because this field i am very new and i don't know how to isolate actinomycetes from medicinal plants. If you send this paper and any another information then i am very happy and also very gratful to you.
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