Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Current Research in Tuberculosis
  Year: 2009 | Volume: 1 | Issue: 1 | Page No.: 1-8
DOI: 10.17311/crt.2009.1.8
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Immunological and Molecular Diagnosis of Mycobacterium tuberculosis Between two Environmentally Different Regions

Abeer E. Abdelwahab

Abstract:
Mycobacterium tuberculosis (M. tuberculosis) is an airborne contagious disease that is transmitted by coughing, sneezing or even talking. Once a person becomes infected, any condition that weakens the immune system can trigger the development of active M. tuberculosis. Exposure to smoke can increase the risk of M. tuberculosis by reducing resistance to initial infection or by promoting the development of active M. tuberculosis in already infected persons. Exposure to the pollutants contained in biomass smoke or other sources of air pollution can weaken the immune system, impair the lungs and make them more susceptible to infection and disease. The association between air pollution and respiratory infections is well understood but the relation between air pollution and tuberculosis is not. So, it is anticipated that the prevalence of M. tuberculosis is positively correlated with environmentally polluted areas. In order to compare the prevalence of M. tuberculosis in two different areas in Alexandria city, industrial area (showing petroleum oil, fertilizer and cement industries) and residential area, golden standard methods [acid fast staining and culture] and molecular assays [Polymerase Chain Reaction technique (PCR) and Enzyme-Linked Immunospot assay (ELISpot)] of M. tuberculosis detection were used. There was a significant increase in the prevalence of M. tuberculosis in the industrial district than the residential one. Molecular tool of M. tuberculosis diagnosis was found to be the best than immunological method. Air pollution produced by petroleum, fertilizer and cement industries most likely has major effect on increasing the prevalence of M. tuberculosis infection. On the other hand, the power of the molecular techniques was confirmed and showed to be more sensitive than the traditional methods and the PCR assay was more accurate than the ELISpot assay.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Electrochemical Technique using Methylene Blue with Pencil Graphite Electrode for Optimum Detection of Mycobacterium tuberculosis DNA
  •    Sensitivity of IS6110, mtp40 and 85B-RNA Based Amplification Assays in the Diagnosis and Treatment Follow up of Pulmonary Mycobacterium tuberculosis
How to cite this article:

Abeer E. Abdelwahab , 2009. Immunological and Molecular Diagnosis of Mycobacterium tuberculosis Between two Environmentally Different Regions. Current Research in Tuberculosis, 1: 1-8.

DOI: 10.17311/crt.2009.1.8

URL: https://scialert.net/abstract/?doi=crt.2009.1.8

COMMENTS
19 March, 2009
kaivaramsarada:
its good to see this abstract, but what about the latent tuberculosis infected people. bcs of the pollution they r prone to T.B or they resist?
23 March, 2009
Abeer Abd El- Wahab, ( the author):
Reply:

Patients with latent tuberculosis are not infectious, and it is not possible to get TB from someone with latent tuberculosis. The main risk is that approximately 10% of these patients will go on to develop active tuberculosis at a later stage of their life.
There are currently two γ-interferon tests available to identify patients with latent tuberculosis:


• ELISPOT (done on my published article)


• QuantiFERON-TB
25 February, 2011
Prof(Dr) Jogenananda Pramanik.MD:
Like several other researchers, Abeer E. Abdelwahab once again confirmed the power of the molecular techniques and showed it to be more sensitive than the traditional methods and the PCR assay was more accurate than the ELISpot assay.However,the Gold standard test for tuberculosis is the age old culture method because PCR assay picks up the DNA fragment of dead bacilli and gives false positive results in some situation.PCR assay is expensive and not available at the resource poor set up in most of the developing or under developed countries and diagnostic delay continues to be a never-ending problem for the clinicians in the field situations. Therefore,during my MD thesis work,I described an innovative culture method to expedite bacilli cultivation in modified solid and liquid media which is proved to be useful for early and accurate diagnosis of tuberculosis in any resource poor setting in peripheral clinics.



References:
10.1136/bmj.326.7395.909
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

Curve Bottom