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  Year: 2011 | Volume: 10 | Issue: 6 | Page No.: 498-505
DOI: 10.3923/biotech.2011.498.505
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High Potential Probiotic Bacillus Species from Gastro-intestinal Tract of Nile Tilapia (Oreochromis niloticus)

K. Chantharasophon, T. Warong, P. Mapatsa and V. Leelavatcharamas

In study to obtain a safe Bacillus probiotic for Nile tilapia (Oreochromis niloticus) cultivation, hemolytic activity on blood agar medium was used in isolation of Bacillus probiotic species from gastro-intestinal tract of Nile tilapia. One hundred and three isolates of Bacillus sp. which showed no hemolytic activity were obtained from 2 sampling sites of the Nile Tilapia net-cage culture farms. Among these 103 isolates, however, there was only 1 isolate, named as Bacillus UBRU4 which showed the inhibitory effect on Aeromonas hydrophila growth. The results of physiological and biochemical test and molecular identification (99.90% identity) showed that Bacillus UBRU4 was similar to Bacillus brevis. This was possibly the first report of isolation of Bacillus brevis in aquaculture. The optimum pH and temperature for Bacillus UBRU4 growth on Tryptic soy broth were 6.5 and 37°C, respectively. The maximum cell numbers of Bacillus UBRU4 in modified broth culture medium was obtained when using the medium contained 30 g L-1 of Nile tilapia commercial feed and 20 g L-1 of molasses. The bioactive compound production of Bacillus UBRU4 showed the growth associated characteristic. Partial purified bioactive compounds by 80% saturated ammonium sulfate could increase the activity to 6,400 AU mL-1. The specific activity of the bioactive compound was increased from 1,298 to 5,807 AU mg-1. These results suggested that the Bacillus UBRU4, thus, could possibly be used as high potential probiotic in Nile tilapia feed.
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How to cite this article:

K. Chantharasophon, T. Warong, P. Mapatsa and V. Leelavatcharamas, 2011. High Potential Probiotic Bacillus Species from Gastro-intestinal Tract of Nile Tilapia (Oreochromis niloticus). Biotechnology, 10: 498-505.

DOI: 10.3923/biotech.2011.498.505






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