
	Biotechnology
	Year: 2011 \| Volume: 10 \| Issue: 3 \| Page No.: 259-266 DOI: 10.3923/biotech.2011.259.266

Whole Cell Based Electrochemical Biosensor for Monitoring Lead ions in Milk

Neelam Verma, Hardeep Kaur and Sachin Kumar

Abstract: Rapid industrialization, urbanization and modern agricultural practices have led to cumulative pollution of environment by heavy metals, thereby deteriorating water and food product quality. Milk and its products are most prone to heavy metal contamination and lead has been considered as a major contaminant in milk. A whole cell electrochemical lead biosensor has been developed using urease producing Bacillus sphaericus isolate (MTCC 5100) which was grown at 30°C for 24 h and then harvested in phosphate buffer saline. Urease enzyme units available in the biomass were 53.87±1.41 IU mL^-1. Whole cells were immobilized at the tip of carbon paste electrode and unutilized NADPH oxidation has been studied to reveal the lead concentration in milk. Preconcentration and specificity has been achieved by lead specific column. Lead analysis has been done at 25°C in 5 min response time. Detection limit achieved is 2.4x10^-3nM well below the permissible limit of lead in milk. The developed method could be used for off line analysis of lead by portable handheld potentiostat.

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How to cite this article:

Neelam Verma, Hardeep Kaur and Sachin Kumar, 2011. Whole Cell Based Electrochemical Biosensor for Monitoring Lead ions in Milk. Biotechnology, 10: 259-266.

DOI: 10.3923/biotech.2011.259.266

URL: https://scialert.net/abstract/?doi=biotech.2011.259.266

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