Submit Manuscript

Biotechnology

Year: 2011 | Volume: 10 | Issue: 2 | Page No.: 203-208
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 295

Authors

R. Pratap Chandran

V.P. Potty

Keywords

Research Article

Different Inducer Molecules and Strains of Agrobacterium rhizogenes on Enhancing Transformation Frequency in Host Plants

R. Pratap Chandran and V.P. Potty
The aim of the present investigation was to find the best Agrobacterium rhizogenes strain, which can induce hairy root initiation faster and the best inducer molecule which can potentiate hairy root induction in host plants. Four different wild strains of A. rhizogenes, namely A. rhizogenes 15834, A4, WS and WR were used for hairy root infection in four host plants (Ipomoea batatas, Solenostemon rotundifolius, Vigna vexillata and Canavalia sp). Agrobacterium rhizogenes strains initiated hairy roots from the host plants without inducer molecules (AS and sugars) to a lesser level. Phenolic compound acetosyringone (AS) and sugars (D-glucose, mannose and galactose) were used to activate the virulence genes of the root inducing (Ri) plasmid which initiates transcription of virulence genes for hairy root induction. AS and sugars activated the virulence genes of the root inducing (Ri) plasmid of A. rhizogenes strains, which further initiated the transfer of T-DNA region to the host plants and enhanced hairy root induction frequency. As enhanced hairy root induction percentage in the entire host plants ranges from 11 to 61.5% and sugars from 16.5 to 39.5%. A. rhizogenes 15834 was found to initiate hairy roots early when compared to other strains of A. rhizogenes.
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *