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Biotechnology
  Year: 2010 | Volume: 9 | Issue: 1 | Page No.: 41-47
DOI: 10.3923/biotech.2010.41.47
 
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The QTLs, Determination of Cocoon Shell Weight Trait in Mulberry Silkworm (Bombyx mori L.)

A.R. Bizhannia, S.Z. Mirhosseini, B. Rabiee and M. Taeb

Abstract:
The silkworm is an important economical insect in sericultural field. Its major production-economic characteristics are polygenic. In this study, three F2 populations (second generation) derived from three cross between three pairs, parents of Lemon Khorasan (as maternal) and 107 (as paternal) lines. we contacted AFLP (amplified fragment length polymorphism) technique for mapping genetic factors or QTLs that effect on Cocoon Shell Weight (CSW) trait. Following it we used 20 selected primer combinations of PstI/TaqI and DNAs were individually extracted to phenol-chloroform method. They digested by two restriction enzymes (TaqI and PstI) and also produced DNA fragments amplified by appropriate adaptors separately. After transferring of DNAs samples on annealed 6% polyacrylamide gels and genotyping of individuals, the linkage maps of each population were drawed. The QTLs for cocoon shell weight trait in LRS = LRT>12.5 (LOD>2.71) threshold level based on permutation test (n = 1000) and using compound interval mapping methods were analyzed and detected 5, 1 and 1 QTLs that were localized on linkage groups 9, 11; 12 and 24 in each studied F2 populations, respectively. The QTLs had different gene effects from over dominance, dominance to partial dominance.
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 RELATED ARTICLES:
  •    Significant Tests of Coefficient Multiple Regressions by using Permutation Methods
  •    Investigation on Improvement Possibility of Resistance, Production and Reproduction Traits in 3P, 2P and P Generations in Three Japanese Pure Lines of Silkworm Bombyx mori L., Using Individual Selection in 3P Generation
How to cite this article:

A.R. Bizhannia, S.Z. Mirhosseini, B. Rabiee and M. Taeb, 2010. The QTLs, Determination of Cocoon Shell Weight Trait in Mulberry Silkworm (Bombyx mori L.). Biotechnology, 9: 41-47.

DOI: 10.3923/biotech.2010.41.47

URL: https://scialert.net/abstract/?doi=biotech.2010.41.47

COMMENTS
29 January, 2010
pinky mohal:
i want more material bcoz this is my seminr topic so plz give me the more information plz plz plz.........
29 January, 2010
Alireza Bizhannia:

Hellow. Please send your questuion and requested information via email ([email protected]) as clear and compeletely english.

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