
	Biotechnology
	Year: 2009 \| Volume: 8 \| Issue: 2 \| Page No.: 212-219 DOI: 10.3923/biotech.2009.212.219

Improved Production of Endoglucanase Enzyme by Aspergillus terreus; Application of Plackett Burman Design for Optimization of Process Parameters

Gahda A. Youssef and Mahmoud M. Berekaa

Abstract: In this study, bagasse was used as substrate for endoglucanase (carboxymethyl-cellulase; CMCase) production using locally isolated Aspergillus terreus and the culture parameters were optimized for enhancing cellulase yield. The fungus showed 0.9 U mL^-1 endoglucanase (CMCase) activity, during growth on basal salts medium at 35^oC, initial pH value of 5.5 and in presence of 5% bagasse as a sole c-source. Preliminary experiments to address the most suitable nitrogen source as well as the optimal substrate (bagasse) treatment revealed that the optimal enzyme activity were 2.1 and 2.43 U mL^-1 in presence of 3 g yeast extract and 1 N HCl or 1 NaOH, respectively. Statistically based experimental design was applied to optimize the production of endogluconase by A. terreus. To evaluate the effect of different culture conditions on the production of CMCase enzyme, Plackett-Burman factorial design was carried out. Twelve variables were examined for their significance on enzyme production. Treated bagasse (T₂), non treated bagasse (NT), K₂HPO₄, NaNO₃, trace elements, KCl, temperature and pH were the most significant factors encourage CMCase enzyme production, whereas treated bagasse (T₃), yeast extract and MgSO₄, were the most significant factors decreasing CMCase enzyme production. The pre-optimized medium showed approximately 4 folds increase in cellulase enzyme production.

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How to cite this article:

Gahda A. Youssef and Mahmoud M. Berekaa, 2009. Improved Production of Endoglucanase Enzyme by Aspergillus terreus; Application of Plackett Burman Design for Optimization of Process Parameters. Biotechnology, 8: 212-219.

DOI: 10.3923/biotech.2009.212.219

URL: https://scialert.net/abstract/?doi=biotech.2009.212.219

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