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Biotechnology
  Year: 2007 | Volume: 6 | Issue: 1 | Page No.: 105-111
DOI: 10.3923/biotech.2007.105.111
 
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Stable Transformation and Recovery of Transgenic Plants by Particle Bombardment in Pinus wallichiana A.B. Jacks (Himalayan Blue Pine)

Ravindra B. Malabadi and K. Nataraja

Abstract:
Pinus wallichiana A. B. Jacks is an important Himalayan pine species predominant in the Northern Himalayan range. We used in vitro embryogenic tissue of mature zygotic embryos as target material for genetic transformation. Regeneration of embryogenic tissue before particle bombardment and long selection period, combined with selective agent after bombardment, led to the production of transformed plantlets. Present results indicate that high levels of transient foreign gene expression can be achieved in pines. The procedure reported here is very simple, efficient and reproducible and is applicable across diverse genotypes of Himalayan blue pine. Expression of positive histochemical GUS activity (39%) in the bombarded embryogenic tissue was observed. PCR analysis of bar transgenes (52%) transformation efficiency indicated successful genetic modifications of P. wallichiana embryogenic tissue by the pAHC25 plasmid, where 50% of the selected plants showed gene integration and expression.
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How to cite this article:

Ravindra B. Malabadi and K. Nataraja , 2007. Stable Transformation and Recovery of Transgenic Plants by Particle Bombardment in Pinus wallichiana A.B. Jacks (Himalayan Blue Pine). Biotechnology, 6: 105-111.

DOI: 10.3923/biotech.2007.105.111

URL: https://scialert.net/abstract/?doi=biotech.2007.105.111

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