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Biotechnology

Year: 2006 | Volume: 5 | Issue: 2 | Page No.: 217-221
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Authors

L. Shantikumar Singh Singh

Indra Baruah

T.C. Bora

Keywords

Research Article

Actinomycetes of Loktak Habitat: Isolation and Screening for Antimicrobial Activities

L. Shantikumar Singh Singh, Indra Baruah and T.C. Bora
A total of 37 actinomycetes with distinct characteristics were isolated from the soil sample collected from the Phoomdi in Loktak lake of Manipur, India. These isolates were screened for their antimicrobial activity. Out of 37 isolates, only 21 showed antimicrobial activity against test microorganisms in primary screening process by spot inoculation technique on agar medium. These 21 putative isolates were then subjected to Submerge culture and their antimicrobial activity was evaluated. Of these 21 isolates, 12 were found to be active against the test microorganisms namely Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Mycobacterium phlei, Candida albicans, Fusarium moniliforme. Most of these active isolates showed to possessed antifungal property. These 12 active isolates were identified to be of the genus Streptomyces. Isolate no. A2D was found to have a broad spectrum of activity against all the tested microorganisms. The antibiotic profile of this isolate underlined its potential as a source of novel antibiotic.
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