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Biotechnology
  Year: 2006 | Volume: 5 | Issue: 2 | Page No.: 148-152
DOI: 10.3923/biotech.2006.148.152
 
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Production and Properties of Thermostable Xylanase by Thermomyces lanuginosus NK-2 Grown on Lignocelluloses

K. Naveen, S.C. Agrawal and P.C. Jain

Abstract:
Thermophilic Thermomyces lanuginosus NK-2, isolated from wheat straw compost, produced 1418 IU mL-1 of thermostable xylanase (EC 3.2.1.8) on Czapek`s basal medium containing wheat bran (2% w/v). The specific activity of NK-2 xylanase was found to be 1107 IU mg-1 of total protein. The fungus could also utilize different  low-value  agricultural  residues  and  produce  xylanase  on  corncobs  (1004 IU mL-1), lentil bran (941 IU mL-1) and groundnut shells (355 IU mL-1). Supplementation of pure xylan (1% w/v) to wheat bran medium enhanced xylanase yield by 39% while other C-supplements repressed it. The xylanase was found to be active over a broad pH (4.0-9.0) and temperature range (25-85°C) and the pH and temperature optima were 5.0 and 6°C, respectively. It showed excellent thermal stability by retaining 83.4% activity after one hour of incubation at 6°C and its half-life at 7°C was 40 min. Presence of β-xylosidase (0.007 IU mg-1) was also noticed while CMCase and α-L-arabinosidase were not detected.
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 RELATED ARTICLES:
  •    Effect of Signal Sequence on the β-xylanase from Thermomyces lanuginosus SKR Expression in Escherichia coli
How to cite this article:

K. Naveen, S.C. Agrawal and P.C. Jain , 2006. Production and Properties of Thermostable Xylanase by Thermomyces lanuginosus NK-2 Grown on Lignocelluloses. Biotechnology, 5: 148-152.

DOI: 10.3923/biotech.2006.148.152

URL: https://scialert.net/abstract/?doi=biotech.2006.148.152

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