Science Alert
 
Blue
   
Curve Top
Biotechnology
  Year: 2003 | Volume: 2 | Issue: 3 | Page No.: 222-240
DOI: 10.3923/biotech.2003.222.240
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Spirulina Species as a Source of Carotenoids and a-Tocopherol and its Anticarcinoma Factors

Hanaa H. Abd El-Baky, Farouk K. El Baz and Gamal S. El-Baroty

Abstract:
In the present study blue-green alga Spirulina platensis and Spirulina maxima were grown in batch culture at different nitrogen and NaCl concentrations, respectively. Both species were found to respond to nitrogen deficiency and high NaCl level by accumulation of large amounts of commercially important chemicals such as carotenoids and tocopherols. The higher carotenoids and α-tocopherol contents was obtained when Spirulina sp grown at low nitrogen level (51 ppm N), with values ranged from 19.82 to 24.1 mg g-1 and from 533.2 to 978.5 μg Kg-1 (dry weight, d.w.), respectively. Whilst, these values in cells grown in free nitrogen medium were ranged from 30.15 to 31.13 mg g-1 and from 960.4 to 1325.7 μg Kg-1 (d. w), respectively. The high performance liquid chromatography (HPLC) elution patter of total caroteniods extracted from S. platensis and S maxima culture contained β-carotene (49.6 to 319.5 μg g-1), lutein (0.06 to17.21 μg g-1), astaxanthin (6.61 to 160.27 μg g-1), zeaxanthin (1.25 to 18.55 μg g-1) and cryptoxanthin (1.41 to 20.13 μg g-1). S. maxima accumulated a large amount of cryptoxanthin and zeaxanthin than that found in S. platensis. The effect of Spirulina species extracts containing various carotenoid compounds and tocopherols on the viability of Ehrlich ascites carcinoma cells (EACC) were evaluated. All algae extracts at different concentration of 200 and 400 ppm significant reduced the cell viability ranged from 89.11 to 5.25%. These extracts did not induce any significant changes in DNA fragmentation of treated EACC compared with untreated cells. But lactate dehydrogenase (LDH) and glutathione-S- transferase (GST) enzyme activities and glutathione level in treated EACC were significantly higher than that in untreated cells. These finding suggest that algae extracts may be reduce cell viability by other mechanism such as membrane lyases instead of apoptosis. Thus, Spirulina extract rich in carotenoids and tocopherols could be used as chemopreventive agents since they are relative non-toxic
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Growth and Total Carotenoid, Chlorophyll a and Chlorophyll b of Tropical Microalgae (Isochrysis sp.) in Laboratory Cultured Conditions
  •    Dichloromethane-methanol Extract from Borassus aethiopum Mart. (Arecaceae) Induces Apoptosis of Human Colon Cancer Ht-29 Cells
  •    The Effects of Dietary Supplementation with Spirulina platensis in Growing Rats
How to cite this article:

Hanaa H. Abd El-Baky, Farouk K. El Baz and Gamal S. El-Baroty, 2003. Spirulina Species as a Source of Carotenoids and a-Tocopherol and its Anticarcinoma Factors. Biotechnology, 2: 222-240.

DOI: 10.3923/biotech.2003.222.240

URL: https://scialert.net/abstract/?doi=biotech.2003.222.240

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 



Curve Bottom