Research Article

**Effects of Plant Growth Regulators on Callus Proliferation, Plantlet Regeneration and Growth of Plantlets of Doritaenopsis Orchid**

Israt Chowdhury, Abu Reza Md. Mahfuzur Rahman, M. Obaidul Islam and S. Matsui

Supplement of 2,4-D and BAP into New Phalaenopsis (NP) medium was not effective for the growth of callus in Doritaenopsis orchid. Among the different combinations of BAP and NAA, 5.0 mg l^-1 BAP + 0.1 mg l^-1 NAA highly enhanced PLB formation from calli. For plantlet initiation from PLBs 0.5 mg l^-1 BAP was suitable and higher concentration was inhibitory. Similar effect to that of BAP was found for NAA (0.5 mg l^-1) and BAP + NAA (0.5 mg l^-1 + 0.5 mg l^-1) in plantlet initiation. For in vitro growth of plantlets, 0.5 mg l^-1 of BAP was the best for leaf and shoot growth compared to that of for BAP and NAA. However, for root growth of plantlets 1.0 mg l^-1 BAP + 1.0 mg l^-1 NAA was the most suitable.

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Israt Chowdhury, Abu Reza Md. Mahfuzur Rahman, M. Obaidul Islam and S. Matsui, 2003. Effects of Plant Growth Regulators on Callus Proliferation, Plantlet Regeneration and Growth of Plantlets of Doritaenopsis Orchid. Biotechnology, 2: 214-221.

DOI: 10.3923/biotech.2003.214.221

URL: https://scialert.net/abstract/?doi=biotech.2003.214.221

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Authors

Israt Chowdhury

Abu Reza Md. Mahfuzur Rahman

M. Obaidul Islam

S. Matsui

Keywords

Research Article

**Effects of Plant Growth Regulators on Callus Proliferation, Plantlet Regeneration and Growth of Plantlets of Doritaenopsis Orchid**

How to cite this article

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