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Biotechnology
  Year: 2002 | Volume: 1 | Issue: 1 | Page No.: 21-27
DOI: 10.3923/biotech.2002.21.27
 
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Spectroscopic Study of Porphyrin Production and Accumulation in Culture of Propionibacterium acnes

M. Idrish Miah

Abstract:
The Gram-positive skin bacterium Propionibacterium acnes was grown semi- anaerobically on Eagle’s medium for 16 days at pH 6.7. Porphyrins were produced and accumulated in culture-grown P. acnes. Fluorescence spectroscopy was used to identify and quantify the amount of porphyrins produced in culture during the growth of the cells. Free base protoporphyrin IX (PPIX) as well as both free base coproporphyrin III (CPIII) and metallocoproporphyrin III (MCPIII) were identified in the culture. The CPIII was the dominant porphyrin species present in the early cultured-cells, however, the production and accumulation of PPIX was found to increase linearly with the age of the culture, which could be used in estimating the age of the culture of P. acnes. This investigation might help to understand efficient photodynamic therapy (PDT) of P. acnes.
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How to cite this article:

M. Idrish Miah , 2002. Spectroscopic Study of Porphyrin Production and Accumulation in Culture of Propionibacterium acnes . Biotechnology, 1: 21-27.

DOI: 10.3923/biotech.2002.21.27

URL: https://scialert.net/abstract/?doi=biotech.2002.21.27

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